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A kind of Shengmaisan alcohol extract with anticancer effect and preparation method thereof

A technology of Shengmai powder and extract, applied in the application field of anti-tumor drugs, can solve problems such as poor safety and toxicity, and achieve the effects of low toxicity, good effect and good treatment effect.

Active Publication Date: 2020-08-14
LANZHOU UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] However, the traditional Shengmaisan water decoction has certain toxicity and poor safety. Therefore, on the basis of alcohol extraction of Shengmaisan, the present invention further separates and purifies it through a macroporous resin. The separated and purified components not only retain The effect of Shengmaisan water decoction on inhibiting the overexpression of ras proto-oncogene is confirmed, and the toxicity of Shengmai powder water decoction is greatly reduced, so the effect is better, and it can be used in the preparation of anti-related malignant tumor drugs that inhibit the overexpression of ras proto-oncogene middle application

Method used

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  • A kind of Shengmaisan alcohol extract with anticancer effect and preparation method thereof
  • A kind of Shengmaisan alcohol extract with anticancer effect and preparation method thereof
  • A kind of Shengmaisan alcohol extract with anticancer effect and preparation method thereof

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Experimental program
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Effect test

Embodiment 1

[0025] The preparation method of embodiment 1 Maisan water decoction

[0026] Materials: Ginseng, Ophiopogon japonicus, and Schisandra were all purchased from the Yellow River Market in Lanzhou

[0027] Preparation method: Soak ginseng, Ophiopogon japonicus, and Schisandra chinensis in distilled water, boil, decoct, centrifuge after constant volume, discard the precipitate, filter the supernatant aseptically, and put it in a refrigerator at 4°C for use. Wherein, the ratio of the raw materials ginseng, Ophiopogon japonicus and Schisandra chinensis is 9:9:6.

Embodiment 2

[0028] The preparation method of embodiment two Shengmaisan ethanol extract

[0029] Materials: Ginseng, Ophiopogon japonicus, and Schisandra were all purchased from the Yellow River Market in Lanzhou

[0030] (1) Preparation method of Shengmaisan extract:

[0031] Ginseng, Radix Ophiopogon japonicus and Schisandra chinensis in a ratio of 9:9:6 are added to soak in absolute ethanol, heated to reflux, filtered, concentrated under reduced pressure to obtain Shengmaisan extract.

[0032] (2) Preparation method of Shengmaisan alcohol extract: Dissolve Shengmaisan extract completely in distilled water, absorb it on a macroporous resin column, and then wash and collect it with ethanol solutions of different concentrations. The ethanol concentration is 30% respectively. , 50%, 70%, 100%. At the same time, the water eluate was collected after being washed with water, and used as a control.

[0033] Wherein, the type of macroporous resin column used is D101 type.

Embodiment 3

[0034] Embodiment Three Shengmaisan alcohol extract improves the effect of Shengmaisan water decoction toxicity

[0035] Culture medium, drugs:

[0036] NGM medium: NaCl 1.5g, K 2 HPO 4 1.3g, KH 2 PO 4 8.5g, peptone 1.4g, agar powder 8.5g, add distilled water to 500mL. After sterilization, add filter-sterilized 500µL cholesterol (5mg / mL, prepared in absolute ethanol), autoclaved 500µL 1mol / L MgSO 4 , 500µL 1mol / L CaCl 2 .

[0037] LB liquid medium: NaCl 1g, peptone 1g, yeast extract 0.5g, add distilled water to 100mL. After dissolving, adjust the pH value to 7.0 with 1M NaOH, and sterilize at high temperature.

[0038] M9 buffer: Na 2 HPO 4 12H 2 O 15.12g, KH 2 PO 4 3g, NaCl 5g, MgSO 4 ·7H 2 O 0.25g, high temperature sterilization.

[0039] S base solution (1L): NaCl 58.5g, K 2 HPO 4 10g, KH 2 PO 4 6g; make up to 1000mL with distilled water, and sterilize at high temperature.

[0040] 1M potassium citrate (1L): citric acid·H 2 O 20g, Potassium Citrate·H...

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Abstract

The invention belongs to the field of traditional Chinese medicines, and relates to a pulse activating powder alcohol extract and application thereof in inhibition of overexpression of ras proto-oncogene, in particular to application in preparation of antitumor drugs for inhibiting overexpression of ras proto-oncogene. A research shows that about 90% of pancreatic cancers, 50% of colorectal cancers, 30%-40% of lung adenocarcinoma and 5%-40% of leukemia are caused by overexpression of the ras proto-oncogene, and Ras protein has become an acknowledged target for screening drugs for resisting associated malignancies. An animal experiment shows that the pulse activating powder alcohol extract can lower overexpression caused after mutation of the ras proto-oncogene in a specificity manner, and does not act on a wild type ras proto-oncogene. Meanwhile, compared with the traditional pulse activating powder decoction, the pulse activating powder alcohol extract is low in fatality rate, does not have teratogenic effect, thus is low in toxicity and good in effect, and is expected to be developed into a new-generation antitumor drug.

Description

technical field [0001] The invention relates to an alcohol extract of Shengmaisan and its application in inhibiting the overexpression of the ras proto-oncogene, in particular to the application in the preparation of an antitumor drug for inhibiting the overexpression of the ras proto-oncogene, and belongs to the field of traditional Chinese medicine. Background technique [0002] Shengmaisan is a famous prescription of traditional Chinese medicine through the ages. It was first recorded in the article "Maimendong" in "Medical Qiyuan Volume 2" written by Zhang Yuanyuan, a physician in the Jin Dynasty; Composed of ginseng, Ophiopogon japonicus, and Schisandra chinensis, it is intended to take "the sweetness of ginseng tonify qi, the source of bitterness and coldness of Maidong (that is, Ophiopogon japonicus) to relieve heat and replenish water, and the acidity of schisandra to cleanse and dry gold". [0003] Shengmaisan was first used to treat the syndrome of excessive sweati...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K36/8968A61P35/00
Inventor 李红玉李孟惠支德娟李洋李莹辉陈晓雨
Owner LANZHOU UNIVERSITY
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