Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Cell preservation liquid and application thereof in protecting megakaryocyte progenitor cell activity

A technology of preservation solution and cells, which is applied in the field of cell culture, can solve the problems that cells are easy to gather together, cells cannot provide nutrition guarantee, and the maintenance time of cell activity is short, so as to achieve good vitality of megakaryotic progenitor cells, protect the vitality of megakaryotic progenitor cells, The effect of maintaining activity

Active Publication Date: 2016-02-03
GUANGZHOU SALIAI STEMCELL SCI & TECH CO LTD
View PDF3 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The existing technology only uses normal saline to preserve megakaryotic progenitor cells, and carries out storage and transportation in ordinary incubators. One of the disadvantages is that only normal saline is used to preserve megakaryotic progenitor cells, and the cells cannot be provided with corresponding nutritional guarantees during storage or transportation. The maintenance time is short, and the cells are easy to aggregate together

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Cell preservation liquid and application thereof in protecting megakaryocyte progenitor cell activity

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Add folic acid for injection to the normal saline for injection with a concentration of 0.9%, and the final concentration is 0.0625mg / ml;

[0037] Human serum albumin is wherein the final concentration of albumin is 5mg / ml;

[0038] The astragalus extract mixture (astragaloside IV: calycosin: formononetin = 1:1:1) was added to the above mixture, so that the final concentration of the astragalus extract was 0.625 μg / ml.

Embodiment 2

[0040] Add folic acid for injection to the normal saline for injection with a concentration of 0.9%, and the final concentration is 0.125mg / ml;

[0041] Add human serum albumin to it so that the final concentration of albumin is 50 mg / ml;

[0042]The astragalus extract mixture (astragaloside IV: calycosin: formononetin = 1:1:1) was added to the above mixture, so that the final concentration of the astragalus extract was 1.25 μg / ml.

Embodiment 3

[0044] Add folic acid for injection to the normal saline for injection with a concentration of 0.9%, and the final concentration is 0.09375mg / ml;

[0045] Human serum albumin is wherein the final concentration of albumin is 27.5mg / ml;

[0046] The astragalus extract mixture (astragaloside IV: calycosin: formononetin = 1:1:1) was added to the above mixture, so that the final concentration of the astragalus extract was 0.9375 μg / ml.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to the field of cell culture, in particular to cell preservation liquid and application thereof in protecting megakaryocyte progenitor cell activity. The cell preservation liquid comprises water, folic acid, albumin, an astragalus extract and sodium chloride. The cell preservation liquid can provide corresponding nutrients for cells in the preservation process, so that the activity of the cells is maintained, and the cells are not likely to be gathered or died. More preservation liquid can be provided for preserving the megakaryocyte progenitor cells, the cell activity can be kept at about 90% within 24 h, the cell preservation liquid is obviously better than a control group in which normal saline or the mixture of normal saline and albumin is adopted, and it proves that the preservation liquid can achieve the effect of well protecting the megakaryocyte progenitor cell activity.

Description

technical field [0001] The invention relates to the field of cell culture, in particular to a cell preservation solution and its application in protecting the vitality of megakaryotic progenitor cells. Background technique [0002] Megakaryocyte progenitor cell (megakaryocyte progenitor cell) is a kind of cell that makes people produce megakaryocyte, which is derived from bone marrow progenitor (CFU-GEMM), referred to as megakaryocyte progenitor cell. Megakaryotic progenitor cells exist in the IL-6R- cell population, while CD34+ cells can be divided into two cell subsets, expressing IL-6R and not expressing IL-6R, IL-6R+ cells can be stimulated to form granule-macrophages, Lymphocytes and granulocytes, while IL-6R- cells can form erythroid and megakaryocytes when given IL-6 / sIL-6R. Hematopoietic stem cells first differentiate into megakaryotic progenitor cells, also known as megakaryotic colony forming units (colonyforming unit-megakaryocyte, CFU-Meg). After the megakaryoc...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): A01N1/02
Inventor 陈海佳王一飞葛啸虎李丽娟
Owner GUANGZHOU SALIAI STEMCELL SCI & TECH CO LTD
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com