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Method of extracting Collybia albuminosa polysaccharide from Collybia albuminosa

A technology of gallinarum and polysaccharides, which is applied in the field of biomedicine, can solve the problems of little extraction research, and achieve the effects of high extraction efficiency, simple process, and high content of active ingredients

Inactive Publication Date: 2016-01-27
GUANGXI NANNING SHENGQIAN TECH DEV CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, domestic and foreign reports on the albumen fungus mainly focus on proteins, and there are few studies on the extraction of the albumen fungus polysaccharides from the albumen fungus.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Weigh 100kg of dried gallinaceous fungus and crush it to below 0.5cm, put it into the extraction tank, add 500L of 0.3% hydrochloric acid solution, and start stirring; open the jacket and steam to heat to 40°C, start timing, extract for 3 hours, and the feed liquid passes through 400 mesh filter bag; after the reflux extraction, the feed liquid passes through a 400-mesh filter bag, the filtrate is added with calcium hydroxide to adjust the pH value to 7.5, and centrifuged; the centrifuged liquid is added with acetic acid to adjust the pH value to 5.0, and centrifuged; The flow rate was added to the 100L macroporous adsorption resin column, and the macroporous adsorption resin was washed with water at a flow rate of 120L / h to remove unadsorbed impurities; Porous adsorption resin to remove impurities with higher polarity; then use 65% ethanol solution as the desorption liquid to wash the macroporous adsorption resin at a flow rate of 50L / h, collect the desorption liquid af...

Embodiment 2

[0032] Weigh 100kg of dried gallinaceous fungus and crush it to less than 0.5cm, put it into the extraction tank, add 300L of 0.25% hydrochloric acid solution, and start stirring; open the jacket and steam to heat to 30°C, start timing, extract for 2 hours, and feed the liquid through 400 mesh filter bag; after the reflux extraction is completed, the feed liquid passes through a 400-mesh filter bag, the filtrate is added with calcium hydroxide to adjust the pH value to 8.0, and centrifuged; the centrifuged liquid is added with acetic acid to adjust the pH value to 4.5, and centrifuged; The flow rate was added to the 100L macroporous adsorption resin column, and the macroporous adsorption resin was washed with water at a flow rate of 100L / h to remove unadsorbed impurities; Porous adsorption resin to remove impurities with higher polarity; then use ethanol solution with a volume fraction of 70% as the desorption liquid to wash the macroporous adsorption resin at a flow rate of 40...

Embodiment 3

[0034] Weigh 100kg of dried gallinaceous fungus and crush it to less than 0.5cm, put it into the extraction tank, add 400L of 0.35% hydrochloric acid solution, and start stirring; open the jacket and steam to heat to 35°C, start timing, extract for 1 hour, and the feed liquid passes through 400 mesh filter bag; after the reflux extraction, the feed liquid passes through a 400-mesh filter bag, the filtrate is added with calcium hydroxide to adjust the pH value to 7.8, and centrifuged; the centrifuged liquid is added with acetic acid to adjust the pH value to 4.8, and centrifuged; The flow rate was added to the 100L macroporous adsorption resin column, and the macroporous adsorption resin was washed with water at a flow rate of 200L / h to remove unadsorbed impurities; Porous adsorption resin to remove impurities with higher polarity; then use 65% ethanol solution as the desorption liquid to wash the macroporous adsorption resin at a flow rate of 60L / h, collect the desorption liqui...

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PUM

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Abstract

The invention discloses a method of extracting Collybia albuminosa polysaccharide from Collybia albuminosa, comprising following steps: (1), extracting Collybia albuminosa at 30-40 DEG C for 1-3 h with hydrochloric acid solution having a mass fraction of 0.25-0.35% to obtain extract; (2), adding a base to the extract to adjust pH to 7.5-8.0, and removing insoluble substances by solid-liquid separation to obtain clarified aqueous solution A; (3), adding an acid to the aqueous solution A to adjust pH to 4.0-4.5, and removing insoluble substances by solid-liquid separation to obtain clarified aqueous solution B; (4), adding the aqueous solution B to macroporous resin columns, removing unadsorbed impurities by washing the macroporous resin column with water; washing macroporous resin with ethanol solution having a volume fraction of 15-20% to remove impurities higher in polarity; using ethanol solution having a volume fraction of 65-70% as a desorption solution to wash the macroporous resin, collecting the desorption solution after washing, and performing reduced pressure concentration and drying to obtain Collybia albuminosa extract. The method has a reasonable process and high extraction efficiency, is simple to operate and is suitable for industrial production.

Description

technical field [0001] The invention relates to a method for extracting the polysaccharide from the albumen fungus, and the invention relates to the field of biomedicine. Background technique [0002] Chicken fir fungus, scientific name Termitornycesalbuminosus, also known as summer solstice fungus, umbrella mushroom, fir bar fungus (Northeast Sichuan), chicken shiitake mushroom, game cock mushroom or game cock, termite mushroom, termite umbrella, yellow chicken fir. One of the four famous fungi, it is a treasure among edible wild fungi. [0003] Gallus fir fungus is the king of wild edible fungi. Its meat is thick and white, crisp and refreshing, fragrant and delicious, rich in nutrition, especially high in protein content. Protein contains more than 20 kinds of amino acids, of which 8 are essential for human body A complete range of amino acids. Chinese ancient dynasties have praised the termite gallinaceous fungus as delicious and nutritious. According to the analysis,...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/07C08B37/00A23L29/30A23L31/00A23L33/125
Inventor 陈先锐
Owner GUANGXI NANNING SHENGQIAN TECH DEV CO LTD
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