Method for extracting dehydrogenated maslinic acid from olive leaves

Abstract

The invention discloses a method for extracting dehydrogenated maslinic acid from olive leaves. The method comprises the following steps: (1) crushing the olive leaves, extracting with methanol at a temperature of 60-64 DEG C to obtain an extracting solution, and performing solid-liquid separation on the extracting solution to obtain a clarified extracting solution; (2) concentrating the clarifiedextracting solution obtained in the step (1) to Brix 8-10%, adding alkali to regulate the pH value to 8.0-9.0, and performing solid-liquid separation to obtain clarified centrifugate; (3) adding an acid into the clarified centrifugate obtained in the step (2) to regulate the pH value to 4.0-5.0, adding cellulase, and maintaining the temperature for 8 hours at 45-55 EDG C so as to obtain a solution; and (4) adding the solution obtained in the step (3) into a macroporous resin column, washing the macroporous resin column with water to remove the adsorbed impurities, washing the macroporous resin column with a desorption solution, collecting the washed desorption solution, performing vacuum concentration, and drying, thereby obtaining the dehydrogenated maslinic acid extract. The method is reasonable in process, high in content of active ingredients and high in extraction efficiency, and is simple in operation and applicable to industrial production.

Description

technical field [0001] The invention belongs to the technical field of biomedicine, in particular to a method for extracting dehydromaslinic acid from olive leaves. Background technique [0002] Olive is native to the coastal areas of the Mediterranean Sea. It was introduced to my country in the early 1960s. It is an important oil and fruit tree species in my country. It has a planting area of ​​1 million mu and an annual output of 500 tons of olive fruit. [0003] Olive leaves are rich in maslinic acid and dehydromaslinic acid, which are pentacyclic triterpene acids widely distributed in nature and have a wide range of biological activities, such as anti-tumor, hypoglycemic, antibacterial, anti-inflammatory, neuroprotective, etc. Good potential for clinical application. [0004] After the olives are harvested every year, a large number of olive leaves are discarded, which is not only a great waste of resources, but also pollutes the environment. This application aims to p...

AI Technical Summary

Problems solved by technology

At present, reports on olive leaves at home and abroad mainly focus on oleuropein, etc., and there is no research on the extraction of dehydromaslinic acid in olive leaves.