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Method for extracting hericium erinaceus polysaccharide from hericium erinaceus

A technology of Hericium erinaceus polysaccharide and Hericium erinaceus is applied in the directions of anti-tumor drugs, drug combinations, allergic diseases, etc., which can solve the problems of less extraction research, and achieve high extraction efficiency, shortened production cycle, and good extraction consistency. Effect

Inactive Publication Date: 2016-01-27
GUANGXI NANNING SHENGQIAN TECH DEV CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, domestic and foreign reports on Hericium erinaceus mainly focus on proteins, and there are few studies on the extraction of Hericium erinaceus polysaccharides.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Weigh 100kg of dried Hericium erinaceus and crush it below 0.5cm, put it into the extraction tank, add 500L of 0.3% hydrochloric acid solution, and start stirring; open the jacket and steam to heat to 40°C, start timing, extract for 3 hours, and feed the liquid through 400 mesh filter bag; after the reflux extraction, the feed liquid passes through a 400-mesh filter bag, the filtrate is added with calcium hydroxide to adjust the pH value to 7.5, and centrifuged; the centrifuged liquid is added with acetic acid to adjust the pH value to 5.0, and centrifuged; The flow rate was added to the 100L macroporous adsorption resin column, and the macroporous adsorption resin was washed with water at a flow rate of 120L / h to remove unadsorbed impurities; Porous adsorption resin to remove impurities with higher polarity; then use 65% ethanol solution as the desorption liquid to wash the macroporous adsorption resin at a flow rate of 50L / h, collect the desorption liquid after washing...

Embodiment 2

[0032] Weigh 100kg of dried Hericium erinaceus and crush it below 0.5cm, put it into the extraction tank, add 300L of 0.25% hydrochloric acid solution, and start stirring; open the jacket and steam to heat to 30°C, start timing, extract for 2 hours, and feed the liquid through 400 mesh filter bag; after the reflux extraction is completed, the feed liquid passes through a 400-mesh filter bag, the filtrate is added with calcium hydroxide to adjust the pH value to 8.0, and centrifuged; the centrifuged liquid is added with acetic acid to adjust the pH value to 4.5, and centrifuged; The flow rate was added to the 100L macroporous adsorption resin column, and the macroporous adsorption resin was washed with water at a flow rate of 100L / h to remove unadsorbed impurities; Porous adsorption resin to remove impurities with higher polarity; then use ethanol solution with a volume fraction of 70% as the desorption liquid to wash the macroporous adsorption resin at a flow rate of 40L / h, col...

Embodiment 3

[0034] Weigh 100kg of dried Hericium erinaceus and crush it below 0.5cm, put it into the extraction tank, add 400L of 0.35% hydrochloric acid solution, and start stirring; open the jacket and steam to heat to 35°C, start timing, extract for 1 hour, and feed the liquid through 400 mesh filter bag; after the reflux extraction is completed, the feed liquid passes through a 400-mesh filter bag, the filtrate is added with calcium hydroxide to adjust the pH value to 7.8, and centrifuged; the centrifuged liquid is added with acetic acid to adjust the pH value to 4.8, and centrifuged; The flow rate was added to the 100L macroporous adsorption resin column, and the macroporous adsorption resin was washed with water at a flow rate of 200L / h to remove unadsorbed impurities; Porous adsorption resin to remove impurities with higher polarity; then use 65% ethanol solution as the desorption solution to wash the macroporous adsorption resin at a flow rate of 60L / h, collect the desorption solut...

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PUM

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Abstract

The invention discloses a method for extracting hericium erinaceus polysaccharide from hericium erinaceus. The method comprises the following steps of (1) extracting hericium erinaceus by a hydrochloric acid solution with the mass fraction being 0.25 to 0.35 percent for 1 to 3h at 30 to 40 DEG C to obtain an extracting solution; (2) adding alkali into the extracting solution to regulate the pH value to be 7.5 to 8.0, and then performing solid-liquid separation to remove insoluble substances to obtain a clear water solution A; (3) adding acid into the water solution A to regulate the pH value to be 4.0 to 4.5, and then performing solid-liquid separation to remove insoluble substances to obtain a clear water solution B; (4) adding the water solution B into a macroporous absorption resin column; firstly using water to wash macroporous absorption resin to remove impurities which are not adsorbed; then using an ethanol solution with the volume fraction being 15 to 20 percent to wash the macroporous absorption resin to remove impurities with high polarity; next using an ethanol solution with the volume fraction being 65 to 70 percent as deabsorption liquid to wash the macroporous absorption resin, collecting the washed deabsorption liquid, and performing vacuum concentration and drying to obtain hericium erinaceus extracts. The method has the advantages that the process is reasonable; the extraction efficiency is high; the operation is simple; the method is suitable for industrial production.

Description

technical field [0001] The invention relates to a method for extracting Hericium erinaceus polysaccharide from Hericium erinaceus, and the invention relates to the field of biomedicine. Background technique [0002] Hericium erinaceus (scientific name: Hericium erinaceus), also known as Hericium erinaceus, Monkey mushroom, Hericium erinaceus, Hericium erinaceus, is named for its shape resembling Hericium erinaceus. [0003] The nutritional content of Hericium erinaceus is very high, and the dry product contains 26.3 grams of protein per 100 grams, which is twice that of shiitake mushrooms. It contains as many as 17 kinds of amino acids, of which 8 are needed by the human body. Every 100 grams of hericium contains 4.2 grams of fat, which is a veritable high-protein, low-fat food, and is also rich in various vitamins and inorganic salts. Hericium erinaceus has the effects of increasing appetite, enhancing the barrier function of gastric mucosa, increasing the transformation ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00A61P37/02A61P35/00
Inventor 陈先锐
Owner GUANGXI NANNING SHENGQIAN TECH DEV CO LTD
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