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Method for extracting pleurotus ostreatus polysaccharide from pleurotus ostreatus

A technology for oyster mushroom and oyster mushroom, which is applied in the field of biomedicine, can solve problems such as lack of research on oyster mushroom extraction, and achieve the effects of shortening production cycle, simple and coherent process, and high extraction efficiency.

Inactive Publication Date: 2016-01-27
GUANGXI NANNING SHENGQIAN TECH DEV CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, reports on Pleurotus ostreatus at home and abroad mainly focus on protein, polysaccharides, etc., and there is no research on the extraction of oyster mushroom in Pleurotus ostreatus.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Weigh 100kg of dried Pleurotus ostreatus and crush it to less than 0.5cm, put it into the extraction tank, add 500L of 0.3% hydrochloric acid solution, and start stirring; open the jacket and steam to heat to 40°C, start timing, extract for 3 hours, and filter the liquid through 400 mesh bag; after the reflux extraction is completed, the feed liquid is passed through a 400-mesh filter bag, the filtrate is added with calcium hydroxide to adjust the pH value to 7.5, and centrifuged; the centrifuged liquid is added with acetic acid to adjust the pH value to 5.0, and centrifuged; Add the flow rate to the 100L macroporous adsorption resin column, first wash the macroporous adsorption resin with water at a flow rate of 120L / h to remove unadsorbed impurities; then wash the macropores with a volume fraction of 15% ethanol solution at a flow rate of 45L / h The adsorption resin removes the impurities with higher polarity; the macroporous adsorption resin is washed with a flow rate ...

Embodiment 2

[0031] Weigh 100kg of dried Pleurotus ostreatus and crush it below 0.5cm, put it into the extraction tank, add 300L of 0.25% hydrochloric acid solution, and start stirring; open the jacket and steam to heat to 30°C, start timing, extract for 2 hours, and filter the material through 400 mesh bag; after the reflux extraction is completed, the feed liquid passes through a 400-mesh filter bag, the filtrate is added with calcium hydroxide to adjust the pH value to 8.0, and centrifuged; the centrifuged liquid is added with acetic acid to adjust the pH value to 4.5, and centrifuged; Add the flow rate to the 100L macroporous adsorption resin column, first wash the macroporous adsorption resin with water at a flow rate of 100L / h to remove unadsorbed impurities; then wash the macropores with a volume fraction of 15% ethanol solution at a flow rate of 30L / h The adsorption resin removes the impurities with higher polarity; the macroporous adsorption resin is washed with a flow rate of 40L / ...

Embodiment 3

[0033] Weigh 100kg of dried Pleurotus ostreatus and crush it below 0.5cm, put it into the extraction tank, add 400L of 0.35% hydrochloric acid solution, and start stirring; open the jacket and steam to heat to 35°C, start timing, extract for 1 hour, and filter the material through 400 mesh bag; after the reflux extraction is completed, the feed liquid is passed through a 400-mesh filter bag, the filtrate is added with calcium hydroxide to adjust the pH value to 7.8, and centrifuged; the centrifuged liquid is added with acetic acid to adjust the pH value to 4.8, and centrifuged; Add the flow rate to the 100L macroporous adsorption resin column, first wash the macroporous adsorption resin with water at a flow rate of 200L / h to remove unadsorbed impurities; then wash the macroporous resin with a volume fraction of 20% ethanol solution at a flow rate of 50L / h The adsorption resin removes the impurities with higher polarity; the macroporous adsorption resin is washed with a flow rat...

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PUM

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Abstract

The invention discloses a method for extracting a pleurotus ostreatus polysaccharide from pleurotus ostreatus. The method comprises the following steps: (1) extracting the pleurotus ostreatus with a 0.25-0.35 percent by mass hydrochloric acid solution at 30-40 DEG C for 1-3 hours to obtain an extracting solution; (2) adding an alkali into the extracting solution to adjust a pH value to 7.5-8.0, and performing solid-liquid separation to remove insoluble substances in order to obtain a clear aqueous solution A; (3) adding an acid into the aqueous solution A to adjust the pH value to 4.0-4.5, and performing solid-liquid separation to remove insoluble substances so as to obtain a clear aqueous solution B; (4) adding the aqueous solution B into a macroporous adsorption resin column, and washing the macroporous adsorption resin column with water to remove non-adsorbed impurities; washing the macroporous adsorption resin column with a 15-20 percent by volume ethanol solution to remove high-polarity impurities; washing the macroporous adsorption resin column with a 65-70 percent by volume ethanol solution serving as a desorption solution, collecting the washed desorption solution, and performing concentration under a reduced pressure and drying to obtain a pleurotus ostreatus extract. The method is reasonable in process, high in extraction efficiency, easy to operate, and suitable for industrial production.

Description

technical field [0001] The invention relates to a method for extracting oyster mushroom from flat mushrooms, and the invention relates to the field of biomedicine. Background technique [0002] Pleurotus ostreatus (scientific name: Pleurotus otreatus; also known as Pleurotus otreatus, Oyster Mushroom, Black Peony Mushroom, also known as Pleurotus in Taiwan) is a species of Pleurotaceae under the order Agaricaceae of Basidiomycota. It is a fairly common gray edible mushroom. [0003] Pleurotus ostreatus is rich in nutrients, and every 100 grams of dry product contains 20-23 grams of protein, and the amino acid composition is complete, and the mineral content is very rich. Chinese medicine believes that mushrooms are warm in nature and sweet in taste. It has the effects of dispelling wind and cold, relaxing tendons and activating collaterals. It is used to treat pain in the waist and legs, numbness in hands and feet, and blocked tendons and collaterals. The oyster mushroom ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23L31/00
Inventor 陈先锐
Owner GUANGXI NANNING SHENGQIAN TECH DEV CO LTD
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