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Kit for quantitative detection of Scl-70 resisting antibody IgG through chemiluminiscence of magnetic particles and preparing method and detection method of kit

An anti-scl-70igg, scl-70 technology, applied in the field of immunological detection, can solve the problems of poor repeatability, low sensitivity, and troublesome operation.

Inactive Publication Date: 2016-01-20
北京贝尔医疗设备有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The patent discloses a membrane strip immunoassay, which can better explain the problems of low sensitivity, narrow linear range, poor repeatability, troublesome operation and long reaction time of the membrane strip immunoassay.

Method used

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  • Kit for quantitative detection of Scl-70 resisting antibody IgG through chemiluminiscence of magnetic particles and preparing method and detection method of kit
  • Kit for quantitative detection of Scl-70 resisting antibody IgG through chemiluminiscence of magnetic particles and preparing method and detection method of kit
  • Kit for quantitative detection of Scl-70 resisting antibody IgG through chemiluminiscence of magnetic particles and preparing method and detection method of kit

Examples

Experimental program
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Effect test

Embodiment 1

[0066] A kit for quantitatively detecting anti-Scl-70 antibody IgG using magnetic particle chemiluminescence, the kit comprising: Anti-Scl-70IgG calibrator, Anti-Scl-70IgG reagent No. 1, Anti-Scl-70IgG reagent No. 2, Anti-Scl-70IgG Magnetic Separation Reagent, Anti-Scl-70IgG Quality Control and Cleaning Solution.

[0067] The Anti-Scl-70IgG calibrator includes 6 liquid calibration bottles with a loading capacity of 0.5mL, and the target concentrations in the liquid calibration bottles are respectively 0, 5, 20, 50, 100, 200RU / mL Anti-Scl- Tris buffer of 70 IgG and bovine serum albumin;

[0068] The Anti-Scl-70IgG reagent No. 1 is a bottle of 5 mL Tris buffer containing biotin-labeled Anti-Scl-70 antigen and bovine serum albumin;

[0069] The Anti-Scl-70IgG reagent No. 2 is a bottle of 15mL Tris buffer containing alkaline phosphatase-labeled goat anti-human polyclonal antibody and bovine serum albumin;

[0070] The Anti-Scl-70IgG magnetic separation reagent is a Tris buffer b...

Embodiment 2

[0117] A preparation method of a kit for quantitative detection of anti-Scl-70 antibody IgG using magnetic particle chemiluminescence comprises the following steps:

[0118] Step 1. prepare described Anti-Scl-70IgG calibrator comprising: Anti-Scl-70IgG calibrator diluent preparation step and Anti-Scl-70IgG calibrator preparation step:

[0119] The preparation step of described Anti-Scl-70IgG calibrator dilution comprises:

[0120] Add 800mL of purified water and 11.2g of Tris to the container, stir and mix evenly, add 8.5g of sodium chloride and 2mL of Proclin300, stir until completely dissolved, adjust the pH value to 7.0-7.5, add 40g of bovine serum white Put the protein into the container, stir until completely dissolved, then adjust the pH value to 7.0-7.5, dilute to 1L with purified water, filter with a 0.2μm filter, and store the obtained dilution of the calibration product at 2-8°C until use;

[0121] The preparation step of described Anti-Scl-70IgG calibrator compris...

Embodiment 3

[0139] On the basis of Example 2, the kit also includes a cleaning solution, the preparation steps of the cleaning solution:

[0140] Add 800mL of purified water, Tris12.1g and NaCl8.5g to a 1L container, stir until completely dissolved; then add 5g of Tween-20 and 5g of Triton100, stir until completely mixed, and adjust the pH value to 7.5 -8.0, constant volume 1L, filter with a 0.2μm filter, and store at a temperature range of 2-8°C. Before the experiment, the cleaning solution was diluted by adding purified water to 100 mL for every 7.5 mL and mixed well before use.

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Abstract

The invention relates to the technical field of immunological detection, in particular to a kit for quantitative detection of a Scl-70 resisting antibody IgG through chemiluminiscence of magnetic particles and a preparing method and detection method of the kit. The kit for quantitative detection of the Scl-70 resisting antibody IgG through chemiluminiscence of the magnetic particles comprises an Anti-Scl-70 IgG calibration sample, a No.1 Anti-Scl-70 IgG reagent, a No.2 Anti-Scl-70 IgG reagent, an Anti-Scl-70 IgG magnetic separation reagent, an Anti-Scl-70 IgG quality control sample and cleaning liquid. The preparing method and detection method of the kit are further disclosed. According to the detection method, based on traditional film strip immunization and an enzyme linked immunosorbent assay adsorption method, sensitivity is improved by 3-5 orders of magnitudes, the linear range is widened by 3-5 orders of magnitudes, and quantitative detection is achieved; the kit has the advantages of being high in sensitivity and specificity, good in accuracy, low in cost, easy and convenient to operate and objective in result judgment, is capable of achieving full-automatic use with the cooperation of a full-automatic chemiluminiscence immune analysis meter, and has wide application prospects.

Description

technical field [0001] The invention relates to the technical field of immunological detection, in particular to a kit for quantitative detection of anti-Scl-70 antibody IgG by using magnetic particle chemiluminescence and a preparation method and detection method thereof. Background technique [0002] The target antigen of anti-Scl-70 antibody is basic non-histone protein, which is the degradation product of DNA topoisomerase Ⅰ, also known as topoisomerase Ⅰ, because the antibody was originally only found in patients with systemic sclerosis, and its antigen The molecular weight is 70000, so it is called anti-Scl-70 antibody. Anti-Scl-70 antibody is regarded as a serum marker antibody for systemic sclerosis, and positive is often closely related to diffuse skin lesions, proximal skin involvement, cardiac involvement, concurrent tumors, and pulmonary interstitial fibrosis, and is considered to be a poor prognosis index. [0003] At present, there are membrane strip immunoas...

Claims

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Application Information

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IPC IPC(8): G01N33/543
CPCG01N33/54326
Inventor 不公告发明人
Owner 北京贝尔医疗设备有限公司
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