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Effective degradation strain for phenanthrene Sphingobium sp. Phe-1 and application thereof

A high-efficiency, strain-based technology, applied in bacteria, water/sludge/sewage treatment, biochemical equipment and methods, etc., can solve the problems of insufficient degradation degree and degradation time, and achieve good degradation advantages, wide application range and loose conditions. Effect

Inactive Publication Date: 2016-01-20
SUN YAT SEN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, the degradation efficiency of these microbial strains to phenanthrene, including the degree of degradation and the degradation time, is still very insufficient.

Method used

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  • Effective degradation strain for phenanthrene Sphingobium sp. Phe-1 and application thereof
  • Effective degradation strain for phenanthrene Sphingobium sp. Phe-1 and application thereof
  • Effective degradation strain for phenanthrene Sphingobium sp. Phe-1 and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0048] Example 1 Screening of Phenanthrene Efficient Degradation Strains

[0049] 1. Soil samples

[0050] The soil samples were collected from the oil refinery in Longtang Town, Qingcheng District, Qingyuan City (N23°36′20.86″N, E113°04′19.34), removed the debris, put them in sealed bags, and stored them in a refrigerator at 4°C.

[0051] Soil suspension preparation: Weigh 10g of soil into 90mL of high-purity water, shake at 30°C and 180rpm for 30min.

[0052] 2. Screening of high-efficiency degradation strains

[0053] (1) Enrichment culture

[0054] Take 0.5 mL of phenanthrene standard solution in a dry and sterilized Erlenmeyer flask, wait for the acetone to volatilize completely, measure 90 mL of inorganic salt liquid and culture it in the Erlenmeyer flask, then measure 10 mL of soil suspension in the Erlenmeyer flask, so that the concentration of phenanthrene is 10 mg / L, incubate at 25°C, 160rpm in the dark for 5 days. Increase the concentration of phenanthrene to...

Embodiment 2

[0082] Example 2 Phenanthrene efficient degradation bacterial strain Sphingobium sp. Study on the Growth Characteristics of Phe-1

[0083] 1. The effect of temperature on the growth of degrading bacteria

[0084] (1) Using phenanthrene as the sole carbon source, the effects of different temperatures on the growth of the degrading strain Phe-1 were explored.

[0085] A certain concentration (the number of cells is about 10 9 / mL) bacterial suspension was inoculated into 20mL inorganic salt medium (50mg / L phenanthrene content) at a ratio of 1%, and cultured on a shaker at 160rpm for 24h. The culture temperatures were 20°C, 25°C, 30°C, 35°C, and 40°C, respectively. Three parallels were performed for each sample, and the OD600 value was measured, and the average value was calculated. Select the optimum cell growth temperature.

[0086] (2) The results are attached Figure 5 shown. From the experimental results, the degrading strain Phe-1 is highly sensitive to temperature...

Embodiment 3

[0101] Embodiment 3 phenanthrene highly efficient degradation bacterial strain Sphingobium sp. Phe-1 Degradation Ability Determination

[0102] 1. Taking phenanthrene as the only carbon source, the concentration of phenanthrene is 100mg / L, inoculate the degrading strain Phe-1 bacterial suspension at 1%, and measure the degrading strain under the optimum conditions (pH 7.0, rotating speed 160rpm, temperature 35°C) Phe-1 degradation curve.

[0103] The specific method is: a certain concentration (the number of cells is about 10 9 / mL) bacterial suspension was inoculated into 20mL inorganic salt medium (phenanthrene content 100mg / L, pH 7.0) at a ratio of 1%, and cultured on a shaker at 160rpm and 35°C for 0, 1, 2, 3, 4, After 5 days, the whole bottle was extracted, and the residual amount of phenanthrene was determined. Three parallels were performed for each sample, the average value was calculated, and the degradation curve of the degrading bacteria was drawn. Control: ad...

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Abstract

The invention discloses an effective degradation strain for phenanthrene Sphingobium sp. Phe-1 and application thereof. The bacterial strain is preserved at the China General Microbiological Culture Collection Center, the preservation number is CGMCC No.11239, and the preservation address is No.3, No.1 West Beichen Road, Chaoyang District, Beijing. The degradation strain Phe-1 has quite good degradation effect on the polycyclic aromatic hydrocarbon such as phenanthrene, and the degradation rate of 100 mg / L phenanthrene can reach 100% in 48 hours at the temperature of 35 DEG C and the pH of 7.0. The degradation strain is an effective degradation strain, and the degradation conditions are soft, the phenanthrene with the concentration less than 200 mg / L can be degraded at the temperature of 20-35 DEG C and the pH of 5-8. Moreover, the degradation strain has a wide applied range and good application prospect, is suitable for using as a restoration bacterial strain for contaminated land, and has a better degradation capability than the other degradation bacteria.

Description

Technical field [0001] The present invention is the field of organic pollutants degradation bacteria.More specific, involving a Philippines efficient degradation strain Sphingobiumsp. PHE-1 and its application. Background technique [0002] Polycontic aromatics (PAHS) is a general durable toxic organic pollutant in the environment.A large number of studies have confirmed that polygon aromatic hydrocarbons have the role of chronic toxicity and carcinogenic, teratogenic, and mutations, which has attracted widespread attention from environmental science workers in various countries.The Fei (Molecular C14H10), as a three-ring aromatic hydrocarbon that is widely distributed in the natural environment, contains a chemical structure that is closely related to the "Sanzhi" in polychic aromatics-K-region and Bay-RegionModular compound.The research on the degradation of the Philippine biological degradation not only helps the elimination of the Philippines in the environment, but also help...

Claims

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Application Information

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IPC IPC(8): C12N1/20C02F3/34B09C1/10C12R1/01
Inventor 王诗忠蔡丹杨秀虹仇荣亮晁元卿
Owner SUN YAT SEN UNIV
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