A simple method for preparing plga microspheres with uniform particle size

Abstract

The invention provides an improved stirring emulsification method for preparing PLGA microspheres with uniform particle size. The particle size distribution of the microspheres prepared by the method is relatively narrow, and the PLGA microspheres with uniform particle size can be obtained with a high yield through simple centrifugal enrichment. The preparation process is as follows: dissolving PLGA in dichloromethane as the oil phase, using the PVA solution as the water phase, dispersing the oil phase into the water phase in a certain proportion, adding several glass beads with a diameter of 2‑8mm to assist emulsification, and then Stirring and emulsification under a magnetic stirrer; PLGA microspheres with uniform particle size were obtained by volatilization of organic solvents, washing, and enrichment by differential centrifugation.

Description

technical field [0001] The invention relates to a preparation process of polymer microspheres, in particular to PLGA microspheres with uniform particle size distribution, and belongs to the technical field of biomedical materials. Background technique [0002] Polylactic acid hydroxylactic acid (PLGA) is a biodegradable polymer material that can eventually degrade into carbon dioxide and water in the body. It is safe, non-toxic and has good biocompatibility. It is regarded as an excellent medical material and has been approved by the United States. Food and Drug Administration (FDA) approved for clinical use. Microsphere carriers prepared from PLGA materials have important uses in the field of biomedicine and have been extensively studied, including drug delivery, antibody preparation, vaccine adjuvants, etc. At present, the FDA has approved a variety of pharmaceutical preparations based on PLGA microspheres to enter the clinic, such as LupronDeport and so on. In 2009, my ...

AI Technical Summary

Problems solved by technology

Stirring emulsification method is relatively cheap and simple, and the particle size is controlled by stirring speed and time, but because the force of the liquid is very uneven during the emulsification process, the formed particle size is very uneven

Although microspheres of comparable size can be enriched to a certain extent by differential centrifugation, the yield of enriched microspheres is very low (<30%) due to the wide particle size distribution of microspheres.

In addition, using this method to prepare small-sized microspheres requires a very high rotational speed. For example, obtaining 1-2 μm microspheres requires a stirring speed of nearly 10,000 rpm, which requires more professional equipment; ultrasonic emulsification is generally used to prepare nano-scale microspheres , not suitable for the preparation of micron-sized microspheres; the spray drying method not only requires special equipment, but also has poor particle size uniformity; the membrane emulsification method obtains PLGA particles with better uniformity, but the cost of professional equipment and consumables is high

Due to the above-mentioned limitations of existing methods, the quality and uniformity of PLGA microspheres prepared in many studies are not good, and the repeatability of the results is poor; while PLGA microspheres with better uniformity can only be completed in more professional laboratories

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