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A kind of indoor culture method of ostracod

A breeding method and ostracod technology, applied in animal husbandry and other fields, can solve the problems of limiting ostracods, etc., and achieve the effect of simple operation and controllable breeding conditions

Inactive Publication Date: 2017-10-27
EAST CHINA NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But so far, there are few reports on the successful laboratory breeding of ostracods, which limits the role of ostracods in research fields such as evolutionary biology.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] Wuou Dou Xingsuke ( Cypridopsis vidua ) indoor breeding method, comprising the following steps:

[0018] (1) Construction of breeding environment

[0019] Take a 1-liter beaker, soak it in 0.03% potassium permanganate solution for 1 day, wash it with tap water, then sterilize it at 121°C and 100KPa for 20 minutes and dry it for use. Wash the 30-mesh fine sand Boil the stone with water and dry it, then add it to the beaker after drying, so that the thickness of the fine stone sand in the beaker is about 1cm. After fully aerating tap water for 5 days, add it into the beaker to make the liquid level equal to the 1000 ml mark on the beaker. Use an air conditioner to control the room temperature at 25°C, and raise the freshwater species ostracod—Wujiu Xingsuke ( Cypridopsis vidua ) into the beaker, place the beaker in a place with better light but no direct sunlight.

[0020] (2) Delivery of nutrients

[0021] A. Add an appropriate amount of calcium chloride solution ...

Embodiment 2

[0026] Accident Lake Hanasuke ( Limnocythere inopinata ) indoor breeding method, comprising the following steps:

[0027] (1) Construction of breeding environment

[0028] Take 5 liters of glassware, soak it in 0.05% potassium permanganate solution for 3 days, wash it with tap water, then sterilize it under high temperature and high pressure and dry it for use. Put the washed 30-mesh fine sand at 121℃ ,Steam sterilization under the condition of 100KPa for 20 minutes; add to the beaker after drying, the amount added is based on covering the bottom of the beaker. Boil tap water for 10 minutes, cool to room temperature, and let sit for at least 1 day before adding to glassware. Add an appropriate amount of sea salt for cultivation to the glassware so that the salinity in the water is controlled at about 6‰. The saltwater species ostracods put into the breeding object—the accidental lake ostracod ( Limnocythere inopinata ), put the glassware into a constant temperature light...

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Abstract

The invention discloses an indoor cultivation method for ostracoda. In indoor conditions, ostracoda is put in a transparent glass ware, aeration or boiled water which has been put for a while is used as cultivation water, clean and high-temperature disinfected fine sandstones are used as a water-bed matrix where the ostracoda can breed and inhabit, chlorella is used as nutrients needed by vital movement of the ostracoda, then the glass ware is put in a place where is sufficient in sunlight but without direct sunlight, the indoor temperature is controlled within the range from 24 to 26 DEG C during cultivation. The ostracoda can normally breed, and the vitality is vigorous in the above condition. The indoor cultivation method for the ostracoda is low in cost, and easy to operate, a technical issue that most of the ostracoda species can hardly be cultivated indoor can be solved, and base condition guarantee can be provided to scientific research on the ostracoda in future.

Description

technical field [0001] The invention relates to the technical field of aquatic animal cultivation, in particular to a method for cultivating miniature crustacean ostracods. Background technique [0002] Ostracods belong to the crustacea of ​​the phylum Arthropoda, and their shell length is generally about 0.5-2.0 mm. They have a double shell formed by the extension of the carapace and cover the entire body. between shells. Ostracods have appeared since the Paleozoic Era. They are an ancient group of crustaceans, and they are still thriving in various water bodies today. They are common small benthic animals in water bodies. The calcareous shells of ostracods are easy to preserve in strata of various geological ages, so they have rich fossil records. There are about 30,000 fossil species recorded so far. An important "information carrier" in such research. In addition, in addition to rich species, small individuals, and easy access to a large number of population specimens...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01K67/033
CPCA01K67/033
Inventor 马相铭禹娜马顺心李德鹏姚建刚
Owner EAST CHINA NORMAL UNIV
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