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SNP genetic markers related to pig fat deposition traits and their application

A genetic marker and pig fat technology, applied in the field of pig genetic marker preparation, can solve the problems of pork quality decline, low intramuscular fat content, low feed conversion efficiency, etc.

Inactive Publication Date: 2017-11-14
湖北天之力优质猪育种有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Generally speaking, Chinese local pig breeds tend to have strong fat deposition ability, which is manifested in high carcass fat content and intramuscular fat content, but slow growth rate and low feed conversion efficiency; Breeding has successfully reduced backfat thickness and increased carcass lean meat percentage, but pork quality has declined, such as low intramuscular fat content (<1.5%), and poor-quality PSE meat (pale, soft meat) , seepage)

Method used

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  • SNP genetic markers related to pig fat deposition traits and their application
  • SNP genetic markers related to pig fat deposition traits and their application
  • SNP genetic markers related to pig fat deposition traits and their application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Example 1 Obtaining of porcine ACADL gene fragments and establishment of polymorphism detection method

[0029] 1. Extraction of pig genomic DNA

[0030] The test pig breeds of the present invention are large white pigs, landrace pigs (foreign blood breeds), Meishan pigs (Chinese local pig blood breeds), and the samples are all obtained from the Hubei Provincial Key Laboratory of Animal Embryo and Molecular Breeding of the Institute of Animal Husbandry and Veterinary Medicine, Hubei Academy of Agricultural Sciences. Provided by the laboratory, it is a commonly used variety. Pig genomic DNA was extracted using a genomic DNA kit produced by Beijing Biotech Biotechnology Co., Ltd. (operated according to the kit instructions), and the specific steps were as follows:

[0031] (1) Collect 20-50 mg of pig ear tissue, cut it into a paste with ophthalmic surgical scissors, put it into a 2 ml centrifuge tube, add 200 μl of lysate TL, and blow it evenly with the tip of a pipette....

Embodiment 2

[0052] Example 2 Detection and verification of polymorphism distribution of molecular markers of the present invention in different pig groups

[0053] The applicant detected the distribution frequency of two polymorphisms in the pig ACADL gene in the three populations of Large White pigs, Landrace pigs and Meishan pigs. The detection results are shown in Table 1: A / T polymorphism distribution at 498bp , the A allele dominates in Chinese local pig breeds, while the T allele dominates in foreign pig breeds, and there are significant differences between Chinese and foreign pig breeds. At the same time, we also detected: in the distribution of A / G polymorphism at 508bp, the A allele dominates in Chinese local pig breeds, while the G allele dominates in foreign pig breeds. There are also significant differences among Chinese and foreign pig breeds.

[0054] Table 1 The distribution results of the alleles of the two SNPs in intron 6 of the ACADL gene in different varieties

[005...

Embodiment 3

[0056] Example 3 Correlation analysis and application of molecular markers cloned in the present invention and pig fat deposition traits

[0057] 1. Construction of haplotype

[0058] Input the genotype data of the two polymorphic sites of all individuals obtained by the PCR-direct sequencing method into the Haploview software (a free download software), and the haplotype of each individual can be calculated, and the difference between the sites can be calculated at the same time. The degree of pairwise linkage disequilibrium between them is expressed by the standardized linkage disequilibrium coefficient D'. It was found that the linkage disequilibrium coefficient D' between the two loci was equal to 1, which was complete linkage disequilibrium. Two haplotypes AA and TG accounted for 38.6% and 61.4% of all allele frequencies respectively, forming three haplotype combinations AA / AA, AA / TG, and TG / TG.

[0059] 2. Association analysis of haplotype combinations and pig producti...

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Abstract

The invention belongs to the technical field of preparation of livestock molecular markers, and concretely relates to an SNP genetic marker correlated to pig fat deposition property and application. A gene fragment which is correlated to pig fat deposition property and is applied as the molecular marker is obtained through cloning pig ACADL gene, the nucleotide sequence of the gene fragment is shown as a figure 3, A / T base substitution is performed at the 498bp position in the sequence, A / G base substitution is performed at the 508bp position, and substantial difference of allele frequency at the two mutation sites exists in pig breeds of China and foreign countries. The invention also discloses a preparation method of the molecular marker and application of the molecular marker to fat deposition property polymorphism detection, and a new marker is provided for marker auxiliary selection of pigs.

Description

technical field [0001] The invention belongs to the technical field of pig genetic marker preparation, and in particular relates to a SNP genetic marker related to pig fat deposition traits and its application. The genetic marker is obtained by cloning the pig ACADL gene. Background technique [0002] In pig breeding work, breeders have always regarded increasing lean meat percentage and reducing backfat thickness as the main breeding goals. Although this breeding method has achieved remarkable results, its genetic selection eventually leads to a decrease in intramuscular fat content, which in turn leads to a decline in pork quality. With the continuous improvement of consumers' requirements for meat quality, genetic research to improve pork quality has become a research hotspot in animal husbandry. [0003] The fat deposition trait is one of the most important economic traits in pig production and breeding. The content of subcutaneous fat, such as the thickness of backfat...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/68C12N15/11C12N15/10
CPCC12Q1/6888C12Q2600/124C12Q2600/156
Inventor 乔木武华玉梅书棋彭先文吴俊静雷彬宋忠旭孙华李良华刘贵生李明波董斌科
Owner 湖北天之力优质猪育种有限公司
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