Rapid propagation method of gold leaf metasequoia tissue culture

Abstract

The invention discloses a rapid propagation method of gold leaf metasequoia tissue culture. In order to solve the problems that gold leaf metasequoia grafting propagation coefficient is low, cuttage and layering propagation nursery stock is prone to aging, and an existing tissue culture method is high in pollution rate, low in propagation coefficient, unstable and the like, the rapid propagation method of gold leaf metasequoia tissue culture comprises the following steps of explant disinfection, primary culture, secondary culture, rooting and hardening-off culture, acclimatization and transplanting, different culture compositions are used at different culture stages, reasonable acclimatization measures are adopted, and therefore a rapid propagation technical system of gold leaf metasequoia tissue culture is built. A regeneration plant obtained through the method is stable in botanical character and good in repeatability, the growth coefficient reaches 4-6, the rooting percentage reaches 72%, the survival rate of transplanting reaches 96%, and large-scale commercialization seedling production can be carried out.

Description

technical field [0001] The invention belongs to the field of plant tissue culture research, and in particular relates to a method for tissue culture and rapid propagation of metasequoia aureus. Background technique [0002] Metasequoia glyptostroboides 'Gold Rush', also known as Golden Fir, is a plant of the genus Metasequoia in the family Sudaceae. It is native to Japan and is a cultivated variety of Metasequoia developed in recent years. The Golden Leaf Metasequoia tree is dignified and grows rapidly. The crown of the tree is golden all the year round, and the leaves are always bright yellow in spring, summer and autumn. Golden leaf metasequoia has low requirements on environmental microclimate and air humidity, strong stress resistance, cold and heat resistance, water and humidity resistance, and easy management. It is a rare large tree with colorful leaves in urban greening. It is widely used in Liaoning, Zhejiang, Sichuan and other places Both have introduced species a...

AI Technical Summary

Problems solved by technology

[0003] At present, in the production of Metasequoia golden leaf, the propagation methods of cutting, grafting and layering are generally used to cultivate seedlings. Cutting propagation is easily affected by cutting substrates, temperature and humidity, and plant growth regulators. Poor, the formed tree has poor uprightness, no obvious dominance at the top, and slow growth; grafting propagation is the main propagation method of Metasequoia golden leaf, but the grafting method is easily restricted by seasons and regions, and can only be carried out in spring and autumn. Complex, low reproduction coefficient, different seedling growth speed, complicated management, not suitable for batch and large-scale reproduction

Therefore, if we can screen out the method of tissue culture and rapid propagation of metasequoia aurea in production, it will not only simplify the breeding process, but also rapidly expand the number of seedlings to meet market demand. There are few reports on the tissue culture of metasequoia aureus at home and abroad. Gong Weihong et al. The study found that the pollution rate of the primary culture was over 60% in spring and autumn, and over 85% in summer, and the proliferation coefficient was only 1.8, which was far lower than the conventional requirement of tissue culture proliferation coefficient of 3.5.