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Preparation for contaminated soil restoration

A technology of contaminated soil and preparations, applied in the field of contaminated soil remediation, can solve problems such as insignificant effect, high cost, and long cycle

Pending Publication Date: 2015-12-23
卢美珍
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

One is engineering treatment, such as digging and replacing soil, but the implementation is complicated, the cost is high and the soil fertility is easy to be reduced
The second is chemical treatment, the effect and cost are moderate, the disadvantage is that it is easy to reactivate
The third is agricultural management, which is easy to operate and low in cost, but the cycle is long and the effect is not significant

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0014] The main components of a contaminated soil remediation agent are strains WH4, strain WH15, strain WH18, strain WH20, charcoal, active organic materials, and vermiculite.

[0015] Among the above microorganisms, the strain WH4 is Bacillus Licheniformis; the strain WH15 is Pseudomonas taiwanensis; the strain WH18 is Streptomyces microflavus; the strain WH20 is Pseudomonas aeruginosa (Pseudomonas Aeruginosa).

[0016] The proportioning of bacterial species contained in this contaminated soil remediation agent is (percentage by weight):

[0017] (1) WH4 bacterial classification: 10%; (2) WH15 bacterial classification: 13%; (3) WH18 bacterial classification: 40%; (4) WH20 bacterial classification: 37%.

[0018] The manufacture method of this soil remediation agent comprises strain cultivation and preparation, and its processing step is:

[0019] (1) the above-mentioned WH4 bacterial classification, WH15 bacterial classification, WH18 bacterial classification, WH20 bacteria...

Embodiment 2

[0025] Using DNAMAN software analysis, according to the amino acid sequence GenBank: WP_041755179.1 whole gene synthesis of the nucleotide sequence WR gene, through amplification to obtain the target fragment, PCR amplification to obtain the target gene WR (simultaneously through multiplex PCR the corresponding mutation Sites were introduced into the gene sequence to obtain different mutant genes), the PCR product was double-digested with restriction endonucleases, connected to the expression vector pHT43 that had also been double-digested, and transferred by plasmids commonly used in the field The method will verify that the successful recombinant plasmid is transformed from Escherichia coli into the WH4 strain Bacillus licheniformis to obtain genetically engineered bacteria that degrade polluted soil. There are 13 types in total.

[0026] These 13 types of transgenic WH4 strains were respectively prepared according to the method of Example 1 and replaced the original WH4 str...

Embodiment 3

[0027] Embodiment 3 effect verification

[0028] The contaminated soil was taken, and the original heavy metal content of the soil was measured, which were Cd45mg / kg; Pb50mg / kg; Cr70mg / kg.

[0029] The preparations prepared in Examples 1 and 2 were used to treat the soil at a treatment rate of 10 g of the preparation / kg of soil for 10 days. Set up a blank control. The results after processing are as follows:

[0030]

[0031] After applying this preparation, the content of various heavy metals in the soil is significantly reduced, and the effect is very remarkable. It has a good application prospect.

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PUM

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Abstract

The invention relates to a preparation for contaminated soil restoration. Viable bacteria are composed of bacillus licheniformis, pseudomonas taiwanensis, streptomyces microflavus and pseudomonas aeruginosa. The invention further provides a production method of the preparation. The preparation has the obvious treatment effect on contaminated soil and high application and popularization value.

Description

technical field [0001] The invention relates to the technical field of contaminated soil remediation, in particular to a preparation for contaminated soil remediation. Background technique [0002] China has become one of the countries with the most serious environmental problems. According to statistics, by the end of the last century, the area of ​​polluted cultivated land in my country had reached more than 20 million hectares, accounting for about one-fifth of the cultivated land area. Every year, 10 million tons of grain production is reduced due to soil pollution, and another 12 million tons of grain are polluted. Among the substances that pollute the soil, heavy metal pollution plays an important role. On March 28, 2011, the joint meeting of nine ministries and commissions including the Ministry of Environmental Protection pointed to the issue of heavy metal pollution in this year's environmental protection special action. Surveys show that in some cities in the Pe...

Claims

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Application Information

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IPC IPC(8): C12N1/20B09C1/10C07K14/32C12R1/10C12R1/38C12R1/385C12R1/465
Inventor 卢美珍
Owner 卢美珍
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