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Preparing method for kadsura coccinea extract and application of kadsura coccinea extract to treating non-alcoholic fatty liver disease

A non-alcoholic, extract technology, applied in the direction of medical preparations containing active ingredients, drug combinations, pharmaceutical formulas, etc., can solve problems such as research reports on the therapeutic effect of non-alcoholic fatty liver, and achieve a significant therapeutic effect , The effect of simple preparation process

Active Publication Date: 2015-12-23
GUANGZHOU LIUSHUN BIO TEC CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But there is no research report on the therapeutic effect of black tiger on non-alcoholic fatty liver

Method used

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  • Preparing method for kadsura coccinea extract and application of kadsura coccinea extract to treating non-alcoholic fatty liver disease
  • Preparing method for kadsura coccinea extract and application of kadsura coccinea extract to treating non-alcoholic fatty liver disease
  • Preparing method for kadsura coccinea extract and application of kadsura coccinea extract to treating non-alcoholic fatty liver disease

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] A preparation method of black tiger extract, comprising the following steps:

[0023] S1. After crushing the root of the black tiger, extract once with 6 times the volume of 50% (v / v) ethanol solution for 1 hour, let the extract cool and filter, and concentrate the filtrate to obtain a thick paste;

[0024] S2. Take the thick paste and suspend it in 2 times the volume of water, repeatedly extract once with 1 times the volume of petroleum ether-ethyl acetate (wherein ethyl acetate accounts for 50%) mixed solvent, combine the organic solvent extraction layers, recover the solvent, and dry. Crush black tiger extract.

[0025] The total content of biphenylcyclooctadiene-type lignans in the black tiger extract prepared in this example was determined to be 64% by ultraviolet-visible spectrophotometry (with Schizandra A as the reference substance).

Embodiment 2

[0027] A preparation method of black tiger extract, comprising the following steps:

[0028] S1. After pulverizing the root of the black tiger, extract twice with 8 times the volume of 70% (v / v) ethanol solution, each extraction for 2 hours, filter the extract after cooling, combine the filtrate, and concentrate to obtain a thick paste;

[0029] S2. Take the thick paste and suspend it in 3 times of water, extract it twice with a mixed solvent of 2 times of petroleum ether-ethyl acetate (wherein ethyl acetate accounts for 80%), combine the organic solvent extraction layer, recover the solvent, dry, and pulverize to obtain Black Tiger Extract.

[0030] The total content of biphenylcyclooctadiene-type lignans in the black tiger extract prepared in this example was measured to be 86% by ultraviolet-visible spectrophotometry (with Schizandra A as the reference substance).

Embodiment 3

[0032] A preparation method of black tiger extract, comprising the following steps:

[0033] S1. After the root of the black tiger is pulverized, extract 3 times with 12 times the volume of 95% ethanol solution, extracting for 3 hours each time, filter the extract after cooling, combine the filtrate, and concentrate to obtain a thick paste;

[0034] S2. Take the thick paste and suspend it in 5 times of water, extract it repeatedly 3 times with a mixed solvent of 3 times of petroleum ether-ethyl acetate (wherein ethyl acetate accounts for 40%), combine the organic solvent extraction layers, recover the solvent, dry, and pulverize to obtain Black Tiger Extract.

[0035] The total content of biphenylcyclooctadiene-type lignans in the black tiger extract prepared in this example was determined to be 55% by ultraviolet-visible spectrophotometry (with Schizandra A as the reference substance).

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PUM

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Abstract

The invention belongs to the technical field of Chinese herb extracting, and particularly discloses a preparing method for kadsura coccinea extract and an application of the kadsura coccinea extract to treating a non-alcoholic fatty liver disease. The preparing method includes the specific steps that S1, after kadsura coccinea is smashed, extracting is carried out one to three times through a 50%-95% ethanol solution with the volume 6-12 times the volume of the kadsura coccinea, each time of extracting is carried out for 1 h to 3 h, filtering is carried out after natural cooling, filter liquid is merged and concentrated, and thick paste is obtained; S2, the thick paste is taken to suspend in water with the volume 1-5 times the volume of the thick paste, extracting is repeated one to three times through a petroleum ether-ethyl acetate mixed solution with the volume 1-3 times the volume of the mixture, organic solvent extraction layers are merged, solvents are recycled, drying and smashing are carried out, and the kadsura coccinea extract is obtained. In the kadsura coccinea extract prepared with the preparing method, the total content of dibenzocyclooctadiene lignan ingredients ranges from 50% to 100%. In addition, it is first found that the kadsura coccinea extract can be used for treating the non-alcoholic fatty liver disease, and the treatment effect is remarkable.

Description

technical field [0001] The invention relates to the technical field of Chinese herbal medicine extraction, more specifically, to a preparation method of black tiger extract and its application in the treatment of non-alcoholic fatty liver. Background technique [0002] Black tiger is the root and vine of Kadsuracoccinea, also known as stinky rice ball, Guoshanlongteng, scarlet southern Schisandra, etc. It is mainly distributed in Guangxi, Guangdong, Yunnan, Guizhou and other places. It is commonly used by folks. It is used in the treatment of chronic gastritis, acute gastroenteritis, rheumatic arthralgia, bruises and fractures. Modern pharmacological research shows that black tiger has pharmacological effects such as anti-cancer, anti-HIV, anti-inflammatory, liver protection, and anti-oxidation, among which lignans and triterpenoids have been reported more. But there is no research report on the therapeutic effect of black tiger on non-alcoholic fatty liver. Contents of t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/79A61K36/57A61P1/16
Inventor 王来友韩亮王凤云何琳
Owner GUANGZHOU LIUSHUN BIO TEC CO LTD
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