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A kit for reducing nnn content in tobacco extract

A technology of extraction solution and kit, which is applied in the field of kits for reducing NNN content in tobacco extract, and can solve the problems that have not yet been reduced.

Active Publication Date: 2021-07-06
SHANGHAI TOBACCO GRP CO LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is currently no biological enzyme that reduces the harmful component NNN in tobacco extract

Method used

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  • A kit for reducing nnn content in tobacco extract
  • A kit for reducing nnn content in tobacco extract

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] Take 0.01g CYP2A6 enzyme reagent (1nmol / L), 1g NADPNa by weight 2 , 12g trisodium citrate, 0.6g magnesium chloride.

[0045] After the temperature of the tobacco extract dropped below 40°C, the above CYP2A6 enzyme system was added to 1.36kg of the tobacco extract, stirred evenly, and the temperature of the reaction solution was kept at 37°C for 24 hours. Wherein, the amount of the CYP2A6 enzyme system applied in the tobacco extract is 1% of the weight of the tobacco extract.

Embodiment 2

[0047] The sample obtained in Example 1 (tobacco extract sample applied with CYP2A6 enzyme system, reacted for 24h) was diluted 20 times, and the component content was determined by high performance liquid phase-triple quadrupole mass spectrometry, and the determination was repeated 5 times , and take the average value. At the same time, a sample of the tobacco extract without the CYP2A6 enzyme system was taken, and the content of the components was determined after performing the same steps. See Table 1 and Table 2 for the test results of NNN content and other components in the applied sample and the control sample. And taking the control group as a reference, calculate the reduction rate of NNN after applying the CYP2A6 enzyme system. In Table 1, the sample obtained in Example 1 is the application group, and the tobacco extract sample without CYP2A6 enzyme system is the control group.

[0048] The specific calculation formula is:

[0049] NNN reduction rate (%) = ((NNN co...

Embodiment 3

[0057] Take 0.01g CYP2A6 enzyme reagent (1nmol / L), 2g NADPNa by weight 2 , 24g trisodium citrate, 1.2g magnesium chloride.

[0058] After the temperature of the tobacco extract dropped below 40°C, the above CYP2A6 enzyme system was added to 1.36kg of the tobacco extract, stirred evenly, and the temperature of the reaction solution was kept at 37°C for 24 hours. Wherein, the amount of the CYP2A6 enzyme system applied in the tobacco extract is 2% of the weight of the tobacco extract. The content of NNN in the treated extract samples decreased significantly, and the rate of decrease was similar to that of Example 1.

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Abstract

The invention relates to the technical field of tobacco resource reuse, in particular to a kit for reducing NNN content in tobacco extract. The invention provides a kit for reducing NNN content in tobacco extract, including CYP2A6 enzyme, NADPNa 2 , magnesium chloride and buffer system. The kit for reducing NNN in the tobacco extract provided by the invention has no influence on other components of the tobacco extract, is non-toxic and harmless, and is an environment-friendly tobacco harm reducer.

Description

technical field [0001] The invention relates to the technical field of tobacco resource reuse, in particular to a kit for reducing NNN content in tobacco extract. Background technique [0002] Tobacco leaves and stems have many other uses besides being used as the main raw material of cigarettes. The excess or defective tobacco stems can be reused as resources, soaked in aqueous solution, and can be used for the production of reconstituted tobacco leaves. After extraction and separation, the monomer or mixture of active ingredients in tobacco can be obtained, which can be used for cigarette flavoring, Production of e-cigarette oil, and use as chemical intermediates and food additives. However, the tobacco extract contains a variety of harmful tobacco components that are difficult to remove. In the process of using the tobacco extract, these harmful components that are difficult to remove are likely to cause environmental pollution and will enter the cigarette together, enda...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A24B15/20
Inventor 刘兴余周骏张杰张晨白若石杨振东
Owner SHANGHAI TOBACCO GRP CO LTD
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