Method for detecting anti-CD19 chimeric antigen receptor T cell effects of inhibiting leukemia cells
A technology of chimeric antigen receptors and leukemia cells, which is applied in the direction of receptors/cell surface antigens/cell surface determinants, cells modified by introducing foreign genetic materials, chemical instruments and methods, etc., can solve the problems of industrialization, Can not guarantee the accuracy of the inhibitory effect of CART cells on tumor cells and other issues, to achieve high accuracy, easy operation, and short time-consuming effects
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Embodiment 1
[0026] Embodiment 1: A method for detecting the inhibitory effect of anti-CD19 chimeric antigen receptor T cells on leukemia cells disclosed in the present invention, taking B-cell leukemia cells as an example, the detection method includes the following steps, (1) expressing anti-CD19 Chimeric antigen receptor T cells and B cell leukemia cells were mixed in equal proportions and then transplanted into the immune deficiency mouse model: equal proportions of cells generally refer to equal proportions of the number of cells, such as anti-CD19 chimeric antigen receptor T 5x10 each for cells and B-cell leukemia cells 6 A cell, the anti-CD19 chimeric antigen receptor T cell preservation number CCTCCNO:C201503, the cell is preserved in the China Center for Type Culture Collection (Wuhan), the address is Wuhan University, No. 299, Bayi Road, Wuchang District, Wuhan City, Hubei Province (opposite to the No. 1 Primary School Attached to Wuhan University), Wuhan University Preservation ...
Embodiment 2
[0030] Embodiment 2: A method for detecting the inhibitory effect of anti-CD19 chimeric antigen receptor T cells on leukemia cells disclosed in the present invention, taking B-cell leukemia cells as an example, the detection method includes the following steps, (1) expressing anti-CD19 Chimeric antigen receptor T cells and B cell leukemia cells were mixed in equal proportions and then transplanted into the immune deficiency mouse model: equal proportions of cells generally refer to equal proportions of the number of cells, such as anti-CD19 chimeric antigen receptor T 5x10 each for cells and B-cell leukemia cells 6 1 cell, anti-CD19 Chimeric Antigen Receptor T cell with the preservation number CCTCCNO:C201503, preserved in the China Center for Type Culture Collection (Wuhan), and the address is Wuhan University Campus (Wuhan University, No. 299, Bayi Road, Wuchang District, Wuhan City, Hubei Province) Opposite to the First Affiliated Primary School of the University), Wuhan Un...
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