Application of partially indica-type paddy rice dominant dwarf mutant
A kind of mutant and partial indica technology, applied in the application field of partial indica rice dominant dwarf mutants, can solve the problems of narrow genetic basis and increased genetic vulnerability of bred varieties, so as to improve lodging resistance and reduce strain high effect
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[0022] A kind of breeding method for rapidly obtaining partial indica type rice dominant dwarf mutant sd-y described in the present invention comprises the following steps:
[0023] Rice Breeding F grown in the Experimental Farm of the Jiangsu Institute of Agricultural Sciences 2 Substitute seed nursery (coded as F 2 009, the combination of weedy rice × Yanhui 269) obtained the indica type dwarf mutant material (plant height about 50cm); the tall stem and dwarf stem of the selfed offspring were seriously separated, and the mutants with the same height and phenotype were selected. Single plant, keep the seeds to continue planting, use the improved M 8 Medium for pollen tissue culture.
[0024] pollen tissue culture
[0025] Induction medium The basic medium used to induce callus is M 8 culture medium, and 30g·L was added to the culture medium -1 Sucrose, 8g·L -1 of agar powder and 2mg·L -1 2,4-D (2,4-phenoxyacetic acid). Sorbitol 5g·L was also added to the induction med...
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