Application of ZmSBP12 gene in regulation and control of drought resistance, plant height and ear height of corn
A technology of drought resistance and genetics, applied in genetic engineering, plant genetic improvement, application, etc., can solve the problems of low selection efficiency and achieve the effects of reducing ear height, improving drought resistance, and reducing plant height
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[0053] Example 1 Obtaining of ZmSBP12 Gene and Construction of Mutant ZmSBP12-OE
[0054] 1. Acquisition and transformation of ZmSBP12 gene
[0055] The total RNA of B73 inbred line V2 stage plants was extracted by Trizol method (refer to the instruction manual of Trizol of Thermo Fisher Reagent Co., Ltd.), and reverse transcribed (refer to the instruction manual of Tiangen Biochemical Technology Reverse Transcription Kit (KR106-02)). Since the ZmSBP12 gene is a maize SQUAMOSA-PROMOTER BINDING PROTEIN-LIKE (SPL) transcription factor regulated by miR156, it is necessary to see the miR156 regulatory site (CATGCTCTCTCTCTTCTGTCA) for modification so that miR156 cannot recognize and cut ZmSBP12.
[0056] The transformation method is as follows: using primers
[0057]6827-F1 / R1: GTTTGGTGTTACTTCTGCAGATGGAGTGGACGGCCCCGAA / GCTGAGCAGGCTCAGGGCATGCTGAAGATCCGCTGCTC;以上述反转录得到的cDNA为模板,进行第一轮PCR扩增得到片段1;利用引物6827-F2 / R2:GCCCTGAGCCTGCTCAGCGCCGGCGCTTGTGGACTGCC TGATT / TGCCACCACCGGATCCATTTATCTGGTTTACA...
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