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Test strip and method for detecting sodium pentachlorophenate

A technology for sodium pentachlorophenate and test strips, applied in the field of test strips for detecting sodium pentachlorophenate, can solve problems such as high cost of capital and personnel input, water pollution, etc., and achieves high sensitivity, simple storage, and guaranteed reliability. Effect

Inactive Publication Date: 2015-12-02
BEIJING KWINBON BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the 1950s and 1960s, my country once used sodium pentachlorophenate in large quantities to kill the host snails of schistosomiasis, and the effect was obvious. However, sodium pentachlorophenate was relatively stable and polluted water bodies.
[0003] GB29708-2013 "National Food Safety Standard Determination of Sodium Pentachlorophenate Residues in Animal Foods" uses gas chromatography-mass spectrometry for the determination of sodium pentachlorophenate residues in animal foods. The instrument method has the advantages of high sensitivity and accurate results. , but the capital and personnel costs are high

Method used

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  • Test strip and method for detecting sodium pentachlorophenate
  • Test strip and method for detecting sodium pentachlorophenate
  • Test strip and method for detecting sodium pentachlorophenate

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] Embodiment 1 detects the composition of the test strip of sodium pentachlorophenate

[0045] 1. Test paper ( figure 1 )

[0046] The test paper is composed of a bottom plate, a sample absorption pad, a reaction film, a water absorption pad, and a protective film;

[0047] The sample absorbent pad 1, the reaction film 2, the water absorbent pad 3 and the protective film 7 are pasted on the bottom plate 6 in sequence, the end of the sample absorbent pad is connected with the reaction film, the end of the reaction film is connected with the water absorbent pad, and the sample absorbent pad The beginning is aligned with the beginning of the bottom plate, and the end of the absorbent pad is aligned with the end of the bottom plate;

[0048] The test paper is pasted with a protective film, and the protective film 7 is covered on the sample absorption pad, which is the detection end, and the word MAX is printed on it ( figure 2 );

[0049] There are a detection line 4 an...

Embodiment 2 Embodiment 1

[0054] The preparation method of test strip described in embodiment 2 embodiment 1

[0055] 1. Preparation of test strips

[0056] The preparation method of this test strip mainly comprises the following steps:

[0057] 1) preparing freeze-dried microporous reagents with sodium pentachlorophenate monoclonal antibody-colloidal gold marker;

[0058] 2) Prepare a reaction membrane with a detection line coated with sodium pentachlorophenate hapten-carrier protein conjugate and a quality control line coated with goat anti-mouse anti-antibody;

[0059] 3) Assemble the reaction film prepared in 2) with the sample absorbent pad, water absorbent pad, protective film, and bottom plate into a test paper;

[0060] 4) Assemble the freeze-dried microporous reagent and test paper prepared in 1) and 3) with sodium pentachlorophenate monoclonal antibody-colloidal gold marker into a test strip.

[0061] The following step-by-step detailed description:

[0062] (1) Preparation of each compon...

Embodiment 3

[0103] The detection of sodium pentachlorophenate in animal tissues such as embodiment 3 pork, chicken, fish, shrimp

[0104] 1. Sample pretreatment

[0105] (1) Take the fat-removed tissue sample and homogenize it in a homogenizer;

[0106](2) Weigh 2g of the homogenized tissue sample into a 15ml polystyrene centrifuge tube, add 1 bottle of sample extractant, vortex for 3min with a vortexer, and centrifuge at room temperature (20-25°C) above 3000rpm for 5min;

[0107] (3) Pipette 3ml of the upper organic phase into a 10ml polystyrene centrifuge tube, and dry it in a water bath at 50-60°C under nitrogen flow or air flow;

[0108] (4) Add 300 μl sample reconstituted solution, vortex for 20 seconds with a vortexer, and wait for detection.

[0109] 2. Test with test strips

[0110] Take out the reagent bucket and test paper bag from the original packaging, take out the required number of microwell reagents and test paper strips, and mark them. Please use it as soon as possibl...

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Abstract

The invention discloses a test strip and a method for detecting sodium pentachlorophenate. The test strip comprises a test paper and a micropore reagent, and the test paper comprises a sample absorption pad, a reaction membrane, a water absorption pad, a protection membrane and a base plate which are connected in order; the reaction membrane comprises a detection line and quality control line, the detection line is coated with a sodium pentachlorophenate semiantigen-carrier protein conjugate, the quality control line is coated with a goat anti-rat antiantibody, and the micropore reagent contains a freeze-dried sodium pentachlorophenate monoclonal antibody-colloidal gold label. The test strip provided by the invention for detecting sodium pentachlorophenate has the advantages of simple and convenience, rapid, visual sense, portability, wide application range, low cost, and easy popularization and usage.

Description

technical field [0001] The invention relates to a test strip and a method for detecting sodium pentachlorophenate, in particular to a colloidal gold test strip for detecting sodium pentachlorophenate, which is particularly suitable for use in animal tissues such as pork, chicken, fish, and shrimp Detection of sodium pentachlorophenate residues. technical background [0002] PentachlorophenoI-Na (PCP-Na for short) is the sodium salt of pentachlorophene, which belongs to organochlorine pesticides. It is usually white needle-like or scale-like crystals, volatile, and has a distinct pungent smell. It is easily soluble in water, with a solubility of 20.8% at 4°C. Its aqueous solution has a strong phenolic odor, is stable in nature, and is not easy to decompose under direct sunlight. Sodium pentachlorophenate is widely used in the world as an efficient and cheap insecticide, antibacterial agent, preservative and herbicide. In the 1950s and 1960s, my country used a large amount o...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/577
Inventor 罗晓琴冯才伟崔海峰万宇平齐三峰何方洋杨昌松彭鸽
Owner BEIJING KWINBON BIOTECH
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