Microscope automatic focusing method, system and stool microscopy microscope
An automatic focus and microscope technology, applied in microscope, installation, optics, etc., can solve problems such as complex images, poor automatic focus effect, and inability to accurately find the best focus position
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[0034] Such as figure 1 As shown, an automatic focusing method for a microscope comprises the following steps:
[0035] S101: Acquire a number of images with a preset interval step in the image focus area, and write position numbers to the images.
[0036] The image focus area is the image area corresponding to 300 steps above and below the reference position of the microscope. In this embodiment, the CCD is driven by the driving device to collect images of the corresponding position at every predetermined interval step, and at the same time, the position numbers are written to the images according to the order of image acquisition. In a specific implementation manner, the driving device may be a stepping motor, and the predetermined interval step is 5 steps.
[0037] S103: Calculate the first sharpness value of the image by using the first Laplacian operator, and store the first sharpness value corresponding to the image according to the position number to obtain a first sha...
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