Microscope automatic focusing method, system and stool microscopy microscope

An automatic focus and microscope technology, applied in microscope, installation, optics, etc., can solve problems such as complex images, poor automatic focus effect, and inability to accurately find the best focus position

Active Publication Date: 2017-09-01
AVE SCI & TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] For complex images such as stool, due to the complexity of the image, the existing auto-focus effect is poor, and it is impossible to accurately find the clearest best focus position

Method used

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  • Microscope automatic focusing method, system and stool microscopy microscope
  • Microscope automatic focusing method, system and stool microscopy microscope
  • Microscope automatic focusing method, system and stool microscopy microscope

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Experimental program
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Embodiment Construction

[0034] Such as figure 1 As shown, an automatic focusing method for a microscope comprises the following steps:

[0035] S101: Acquire a number of images with a preset interval step in the image focus area, and write position numbers to the images.

[0036] The image focus area is the image area corresponding to 300 steps above and below the reference position of the microscope. In this embodiment, the CCD is driven by the driving device to collect images of the corresponding position at every predetermined interval step, and at the same time, the position numbers are written to the images according to the order of image acquisition. In a specific implementation manner, the driving device may be a stepping motor, and the predetermined interval step is 5 steps.

[0037] S103: Calculate the first sharpness value of the image by using the first Laplacian operator, and store the first sharpness value corresponding to the image according to the position number to obtain a first sha...

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Abstract

The invention provides an automatic focusing method and system of a microscope and a microscope for stool microscopy. The method includes: collecting a number of images with a preset interval step in the focus area of ​​the image, and writing a position number for the image; using the first Laplace The operator calculates the first sharpness value of the image and stores the first sharpness value corresponding to the image according to the position number to obtain the first sharpness value set; according to the first sharpness value set, the inflection point information, the first longest inflection point and the The image of the first maximum sharpness value; according to the position number of the image of the first maximum sharpness value, and the relationship between the position number of the image of the first maximum sharpness value and the first longest inflection point, the corresponding value of the first focus position is obtained; Comparing the value corresponding to the first focus position with zero; if the value corresponding to the first focus position is greater than zero, then the first focus position is an effective best focus position. This method can quickly find the best focus position in more complex images.

Description

technical field [0001] The invention relates to the field of automatic focusing of microscopes, in particular to an automatic focusing method and system of a microscope and a stool microscopy microscope. Background technique [0002] Most of the existing automatic focusing of microscopes is aimed at images that are not very complicated, such as urine. By using the focus evaluation function to automatically search for the focus point, for example, improve on the basis of the traditional mountain climbing algorithm to construct an improved automatic focus search Algorithm for autofocus. [0003] For complex images such as stool, due to the complexity of the image, the existing auto-focus effect is poor, and it is impossible to accurately find the clearest best focus position. Contents of the invention [0004] Based on this, it is necessary to provide an automatic focusing method and system for a microscope capable of effectively finding the best focus position in a complex...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G02B21/00G02B7/36
Inventor 丁建文梁光明王职军
Owner AVE SCI & TECH CO LTD
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