Rice waterlogging stress response RS1 gene and application thereof
A flooding stress response and gene technology, applied in the rice flooding stress response RS1 gene and its application field, can solve the problems of rice photosynthesis inhibition, crop failure, and yield reduction, and achieve obvious effects of flooding resistance and reduce food safety. Concerns, effects of improving flood resistance
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[0016] The working principle of the present invention is as follows:
[0017] Clone the RS1 gene into the pCAMBIAubi1300 transformation vector, introduce it into Agrobacterium, transform the gene into rice recipient cells through the Agrobacterium-mediated method, and then obtain transgenic plants by tissue culture and other technical means.
[0018] 1. Acquisition of rice RS1 gene
[0019] The rice inbred line M202 and its near-isogenic line M202 (Sub1A) with good growth conditions were selected to be flooded for 0 and 5 days, respectively, and total RNA was extracted from the leaves of each treatment. Part of the obtained RNA samples was analyzed by Shenzhen Huada Genomics Co., Ltd. for RNA-seq; the remaining total RNA from each treatment was reverse-transcribed to synthesize the first strand of cDNA. The results of RNA-seq found that the expression of a gene was greatly increased under the flooding treatment, indicating that the expression of the gene was induced by floodi...
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