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Group of genes for tumor molecular subtyping and application thereof

A technique of molecular typing and genetics, applied in the field of cancer diagnosis and molecular biology, which can solve the problems of limited types of cancer and sample size

Active Publication Date: 2015-11-25
HANGZHOU YUANQING BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, a few tumor markers are basically used, which can only be used for the subtype classification of a single tumor
Moreover, the research on these tumor markers is often based on a certain amount of experimental data, and the types of cancers involved and the sample size are relatively limited.

Method used

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  • Group of genes for tumor molecular subtyping and application thereof
  • Group of genes for tumor molecular subtyping and application thereof
  • Group of genes for tumor molecular subtyping and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0142] Training set sample collection and processing:

[0143]The present invention analyzes the clinical data of cancer patients with a large sample size and their biological sample data, including the corresponding clinical data and expression profile data of 5,800 cancer patients with 22 types of tumors from all over the world. Among them, there were 55 cases of adrenal cancer, 446 cases of brain cancer, 542 cases of breast cancer, 113 cases of cervical cancer, 439 cases of colorectal cancer, 262 cases of endometrial cancer, 530 cases of gastroesophageal cancer, 254 cases of head and neck cancer, kidney cancer 256 cases, 222 cases of liver cancer, 285 cases of lung cancer, 366 cases of lymphoma, 163 cases of melanoma, 100 cases of mesothelioma, 209 cases of neuroendocrine tumors, 225 cases of uterine cancer, 134 cases of pancreatic cancer, 458 cases of prostate cancer, 169 cases of sarcoma There were 136 cases of testicular cancer, 238 cases of thyroid tumor, and 198 case...

Embodiment 2

[0152] Validation set test:

[0153] In this example, the inventor analyzed the high-throughput sequencing data of 9626 tumors, including 79 cases of adrenal cancer, 708 cases of brain cancer, 1218 cases of breast cancer, 310 cases of cervical cancer, 434 cases of colorectal cancer, intrauterine cancer 201 cases of membrane cancer, 196 cases of gastroesophageal cancer, 566 cases of head and neck tumors, 1020 cases of kidney cancer, 469 cases of liver cancer, 1130 cases of lung cancer, 48 cases of lymphoma, 554 cases of melanoma, 87 cases of mesothelioma, neuroendocrine tumors 187 cases, 266 cases of uterine cancer, 183 cases of pancreatic cancer, 187 cases of prostate cancer, 265 cases of sarcoma, 156 cases of testicular cancer, 572 cases of thyroid tumors, 427 cases of urinary system tumors. The tissue source was identified by the classification model, and compared with the tumor tissue source recorded in the clinical and pathological data, the average coincidence rate was ...

Embodiment 3

[0157] Screening of 96 specific genes:

[0158] The inventors relaxed the screening criteria of the F test to a P value of less than 0.001, and further obtained an additional 58 genes. These genes were differentially expressed in 22 primary tumors, and the difference was statistically significant. The 58 genes were combined with the 38 genes in Example 1 to form a 96-gene set.

[0159] Table 3: 58-gene set

[0160]

[0161]

[0162] Construction of 96-gene classification model:

[0163] Based on the expression patterns of 96 specific genes in 5,800 tumor samples, the inventors used the support vector machine algorithm to establish a classification model to distinguish the tissue origin and cancer type of tumor samples. For each sample to be tested, the model calculates the similarity score between the gene expression pattern of the sample and the 22 cancer types in the database, and determines the most likely tissue source of the sample according to the principle ...

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Abstract

The invention discloses a group of genes for tumor molecular subtyping. The group of genes includes 38 genes such as an ACPP gene and an AGR2 gene. Moreover, the invention further discloses a kit for tumor molecular subtyping and application of the kit. The group of genes is beneficial to the recognition of the tissue sources of tumors. After a tumor sample is obtained, the tissue source and cancer type of the tumor sample can be judged objectively and accurately through detection and conjoint analysis of the gene group, so that a patient can be treated specifically.

Description

technical field [0001] The invention relates to the fields of cancer diagnosis and molecular biology, as well as the clinical application of diagnosis technology. In particular, the present invention relates to a group of genes for molecular typing of tumors and their applications. The invention also relates to a kit for molecular typing of tumors. Background technique [0002] Tumor is one of the important diseases that threaten human health. According to the "2012 China Cancer Registration Annual Report", one person is diagnosed with cancer every 6 minutes nationwide, 8,550 people are diagnosed with cancer every day, and one person in every seven to eight people dies of cancer. The incidence and death of malignant tumors in my country are on the rise. There are about 3.5 million new cancer cases and about 2.5 million cancer-specific deaths every year. It is estimated that by 2020, the total number of cancer deaths in China will reach about 3 million and the total number...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/11C12Q1/68C40B40/06
Inventor 徐清华杜祥王奇峰陈金影杨扬
Owner HANGZHOU YUANQING BIOTECH CO LTD
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