Method for detecting variety of lactic acid bacteria strain through common or fluorescent quantitation PCR
A fluorescence quantitative, lactic acid bacteria technology, applied in microorganism-based methods, microbial determination/inspection, biochemical equipment and methods, etc., can solve problems such as detection and identification difficulties, and achieve improved detection capabilities, simple operation, and improved detection efficiency. Effect
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[0019] The present invention will be described in detail below in conjunction with specific embodiments.
[0020] A method for detecting the species of lactic acid bacteria by common or fluorescent quantitative PCR, comprising the steps of: a, first downloading all known bacteria-related database information from NCBI, and constructing a local database; b, finding 4 species of lactic acid bacteria Lactobacillus casei, Lactobacillus delbrueckii, Lactobacillus fermentum and Lactobacillus plantarum The data of each genus is searched for the same sequence of each genus through sequence comparison; c, the same sequence of each genus is compared with the blast database of all bacteria, and the unique sequence of this genus is found; d, for The sequence design primer that finds out in step c, carry out electronic PCR in bacterial genome, preliminary screening specific primer; E, carry out PCR detection in positive sample and negative control with the specific primer that screens out...
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