A method for constructing a binary complex bacterial group for biodegradation in wastewater

A binary composite and biodegradable technology, applied in microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve problems such as difficult adaptation and unpredictability, and achieve easy implementation, clear ratio, and stable community structure. Effect

Inactive Publication Date: 2018-09-07
NORTHEAST DIANLI UNIVERSITY
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Problems solved by technology

Commercialized composite flora bacteria species and ratio are fixed, it is difficult to meet the needs of wastewater treatment containing a variety of pollutants in production practice; in addition, it often takes a long period of time to construct a composite flora using the screened strains. Acclimatization and repeated rationing experiments to obtain the optimal ratio of strain compounding, and whether the optimal ratio determined under laboratory conditions can maintain a stable community structure for a long time and form a good mutualistic symbiotic relationship after being added to the actual polluted site is difficult foreknowledge

Method used

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  • A method for constructing a binary complex bacterial group for biodegradation in wastewater
  • A method for constructing a binary complex bacterial group for biodegradation in wastewater
  • A method for constructing a binary complex bacterial group for biodegradation in wastewater

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Embodiment 1

[0047] Embodiment 1: The method for constructing the binary composite flora for phenol biodegradation in waste water of the present invention

[0048] Alcaligenes faecalis and Acinetobacter calcoaceticus were selected. Alcaligenes faecalis and Acinetobacter calcoaceticus were sourced from the China General Microorganism Culture Collection and Management Center. The strain numbers were CGMCC No.1.2098 and CGMCC No.1.6186, for simplicity , and the two strains were abbreviated as PR1 and PR2, respectively. The strains stored on the slant of nutrient broth agar were respectively inoculated into 100 mL of beef extract peptone liquid medium, placed in a shaker at 30 ° C and 150 rpm for 24 h to activate the strains. Take 10 mL of the culture solution after the activation of the strain and inoculate it into 100 mL of the acclimatization liquid medium containing phenol, place it in a shaker at 30°C and 150 rpm for shaking culture, and carry out the acclimatization of the strain. After...

Embodiment 2

[0061] Embodiment 2 The binary composite flora construction method for aniline biodegradation in wastewater of the present invention

[0062]Pseudomonas putida and Acinetobacter calcoaceticus were selected. Pseudomonas putida and Acinetobacter calcoaceticus were sourced from China General Microorganism Culture Collection and Management Center, and the strain numbers were CGMCC No.1.1003 and CGMCC No.1.3615 respectively. For simplicity, the two strains are referred to as BA1 and BA2 respectively. The strains stored on the slant of nutrient broth agar were respectively inoculated into 100mL beef extract peptone liquid medium, placed in a shaker at 30°C and 150rpm for 24h to activate the strains. Take 10 mL of the culture solution after the activation of the strain and inoculate it into 100 mL of the acclimatization liquid medium containing aniline, place it in a shaker at 30° C. and 150 rpm for shaking culture, and carry out the acclimatization of the strain. After 36 hours, ta...

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Abstract

The invention relates to construction methods of binary complex microorganisms for biodegradation in wastewater. The construction methods comprise a phenol construction method and an aniline construction method, wherein each of the construction methods comprises steps of selecting bacterial strains, activating the bacterial strains, domesticating the bacterial strains, storing the bacterial strains, observing colonial morphology, preparing a bacterium suspension, preparing a mixed bacteria suspension of different bacterial strains with the initial proportions, and selecting the optimal proportion of the binary complex microorganisms. On the basis of the two bacterial strains having different colonial characteristics and having degrading capacity for the same organic pollutant, thalli are inoculated into wastewater containing target organic pollutant at different ratios, under specified reaction condition and after specified biochemical reaction time, the degrading condition of the bacterial strains for the target pollutant at different inoculation proportions is determined, meanwhile, colony-forming units of two bacteria in the culture solution at the reaction endpoint time are measured, and finally the proportion of the binary compound microorganisms for biodegrading the target pollutant is determined according to the pollutant degrading efficiency and the calculating result for the relative colony-forming units of the two bacterial strains.

Description

technical field [0001] The invention belongs to the field of waste water biological treatment, and relates to a method for constructing a binary compound bacterial group for biodegradation in waste water. Background technique [0002] In recent years, with the rapid development of the chemical industry, the development and application of new technologies and materials have made wastewater contain a large amount of organic pollutants. Due to the complexity of the structure and biological strangeness of these substances, the types and numbers of indigenous microorganisms that can degrade and transform such pollutants in the polluted environment are relatively small, and there are even no indigenous microorganisms that can degrade some special pollutants. Therefore, traditional wastewater It is difficult to remove them effectively by biochemical treatment. These refractory organic pollutants will eventually be fed back into water, soil and the atmosphere in the form of secondar...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C02F3/34C12R1/05C12R1/01C12R1/40C02F101/38C02F101/34
Inventor 郭静波姜丽杰王伟卓刘明伟
Owner NORTHEAST DIANLI UNIVERSITY
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