Preparation method and application of Shenmei Yangwei granule
A granule and stomach-nourishing technology, which is applied in the field of preparation of Shenmei Yangwei granules, can solve the problems of rough technology, backwardness, and large dosage of patients
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Embodiment 1
[0011] Example 1: Get 140g of Radix Ginseng, 105g of Safflower, 175g of Dandelion, 175g of Radix Paeoniae Alba, 117g of Hawthorn, 105g of Curcuma, 140g of Salvia Miltiorrhiza, 117g of Angelica, 35g of Black Plum, 70g of Echinacea, 70g of Glycyrrhizae, add 8 times the amount of distilled water to extract two Once, each time for 1.5 hours, combine the extracts, concentrate to 0.4ml / g crude drug, add ethanol to make the alcohol content reach 80%, let it stand for 8 hours, filter with suction, take the supernatant, and pass the drug solution through D1010 macropore adsorption Resin column, washed with water until colorless, eluted with 90% ethanol, collected eluate 10BV, evaporated to dryness to obtain part A; concentrated the washing liquid after passing through the macroporous resin, adjusted the pH value of the liquid to 2, and applied the 732 type Cationic exchange resin, washed with water until colorless, eluted with 40% ethanol containing 3% ammonia water, collected the eluat...
Embodiment 2
[0012] Example 2: Experimental Research Data of Shenmei Yangwei Granules Inhibiting the Proliferation of Human Prostate Cancer Cell DU145 Cells
[0013] 1 Experimental materials
[0014] 1.1 The cell line used in the experiment: human prostate cancer cell DU145 cells, the laboratory cell bank of Nanjing Tongrentang Pharmaceutical Co., Ltd., DMEM+10%FBS conventional culture.
[0015] 1.2 Experimental drug: Research drug: Shenmei Yangwei granule of the present invention: prepared according to the method in Example 1. Medicinal solution stock solution: Weigh 100mg of Shenmei Yangwei Granules, dissolve in 5ml of absolute ethanol, filter through a 0.2μm filter, aliquot into 500μl doff tubes, store at -20°C, and filter absolute ethanol through a 0.2μm filter for the control group for.
[0016] 1.3 Experimental reagents: DMEM (GIBCO Cat.No.12100-061 Lot.No.758137); fetal bovine serum (Zhejiang Tianhang Biotechnology Co., Ltd. Lot.No.100419); NaHCO3 (Shanghai Jiuyi Chemical Reagent ...
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