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A kind of method utilizing rubber grass blade to regenerate plant

A technology for regenerating plants and rubber grass, applied in the field of plant cell engineering, can solve problems such as unsatisfactory induction effect, and achieve the effects of short cycle, high reproduction coefficient and high transplant survival rate

Active Publication Date: 2017-05-17
RUBBER RES INST CHINESE ACADEMY OF TROPICAL AGRI SCI +3
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Aiming at the defects in the prior art that the leaves of rubber grass induce callus and regenerate plants, the induction effect is not ideal, the present invention provides a method for using rubber grass blades to regenerate plants

Method used

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  • A kind of method utilizing rubber grass blade to regenerate plant
  • A kind of method utilizing rubber grass blade to regenerate plant
  • A kind of method utilizing rubber grass blade to regenerate plant

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Effect test

Embodiment 1

[0034] Present embodiment relates to a kind of method utilizing rubber grass blade regeneration plant, and its concrete steps are as follows:

[0035] 1) Take young rubber grass leaves, clean the surface with tap water, transfer the cleaned leaves to the ultra-clean workbench and place them in a sterile culture bottle, add 70% alcohol to disinfect the surface for 1 min, and then use Wash 2-3 times with sterile water, then change to a sterile culture bottle, add 15% hydrogen peroxide to sterilize for 7 minutes, then rinse with sterile water 5-6 times, after cleaning, put the leaves on a plate with filter paper Drain the water on the surface and set aside;

[0036] 2) Cut the leaves into about 1-2cm 2 Explants of the same size were inoculated into callus induction medium (see figure 1 ), the culture temperature is 23±2°C, the light intensity is 2000-2500 Lux, and the light is 12 hours a day. The callus induction medium is: MS+2.0mg / L 6-BA+0.5mg / L IAA, and add 30g / L sucrose, 7...

Embodiment 2

[0043] Present embodiment relates to a kind of method utilizing rubber grass blade regeneration plant, and its specific steps are as follows:

[0044] 1) Take young rubber grass leaves, clean the surface with tap water, transfer the cleaned leaves to the ultra-clean workbench and place them in a sterile culture bottle, add 70% alcohol to disinfect the surface for 1 min, and then use Wash 2-3 times with sterile water, then change to a sterile culture bottle, add 15% hydrogen peroxide to sterilize for 7 minutes, then rinse with sterile water 5-6 times, after cleaning, put the leaves on a plate with filter paper Drain the water on the surface and set aside.

[0045] 2) Cut the leaves into about 1~2cm 2 The explants of different sizes are inoculated into the callus induction medium, the culture temperature is 23±2° C., the light intensity is 2000-2500 Lux, and the light is 12 hours a day. The callus induction medium is: MS+1.8mg / L 6-BA+0.4mg / L NAA, with 25g / L sucrose, 6g / L agar,...

Embodiment 3

[0052] Present embodiment relates to a kind of method utilizing rubber grass blade regeneration plant, and its specific steps are as follows:

[0053] 1) Take young rubber grass leaves, clean the surface with tap water, transfer the cleaned leaves to the ultra-clean workbench and place them in a sterile culture bottle, add 70% alcohol to disinfect the surface for 1 min, and then use Wash 2-3 times with sterile water, then change to a sterile culture bottle, add 15% hydrogen peroxide to sterilize for 7 minutes, then rinse with sterile water 5-6 times, after cleaning, put the leaves on a plate with filter paper Drain the water on the surface and set aside;

[0054] 2) Cut the leaves into about 1-2cm 2 The explants of different sizes are inoculated into the callus induction medium, the culture temperature is 23±2° C., the light intensity is 2000-2500 Lux, and the light is 12 hours a day. The callus induction medium is: MS+2.2mg / L6-BA+0.6mg / L IAA, add 35g / L sucrose, 8g / L agar, a...

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Abstract

The invention relates to a method for regenerating plants with Taraxacum kok-saghyz Rodin leaves. The method comprises steps as follows: the pretreated Taraxacum kok-saghyz Rodin leaves are sequentially subjected to callus induction, callus subculture, adventitious bud induction and rooting culture. With the adoption of the induction method, the propagation coefficient is high, browned and albino seedlings are avoided, the regenerated plants are robust, root systems are well developed, the transplantation survival rate is high, and a large number of regenerated plants can be obtained within a short time.

Description

technical field [0001] The invention belongs to the field of plant cell engineering, and in particular relates to a method for regenerating plants by utilizing leaves of rubber grass. Background technique [0002] Rubber grass (Taraxacum kok-saghyz Rodin), also known as Russian dandelion (Russiandandelion), is a perennial herb of the genus Taraxacum in the family Asteraceae, native to Kazakhstan, Europe, and Xinjiang, China. The roots of rubber grass contain high-quality rubber, which can reach more than 20% (perennial dry weight), and its industrial use value is not inferior to that of Hevea brasiliensis rubber. Moreover, rubber grass has a short growth cycle, strong adaptability, and is suitable for mechanized planting. The advantages of harvesting and harvesting make it a promising rubber substitute crop for Hevea brasiliensis. In addition to rubber, inulin can also be extracted from the roots and leaves of rubber grass, and the residue after rubber extraction can be pro...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
Inventor 覃碧王锋张立群刘实忠杨玉双甘霖高玉尧徐建欣
Owner RUBBER RES INST CHINESE ACADEMY OF TROPICAL AGRI SCI
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