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Anticoagulant blackberry seed polysaccharides, and extraction separation method and application thereof

A separation method, technology of blackberry seeds, applied in medical preparations containing active ingredients, blood diseases, medical formulas, etc., can solve problems such as necrosis and tissue ischemia

Active Publication Date: 2015-11-11
HENAN UNIVERSITY
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  • Abstract
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  • Claims
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Problems solved by technology

Once blocked, tissue ischemia and necrosis will occur, resulting in serious consequences

Method used

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  • Anticoagulant blackberry seed polysaccharides, and extraction separation method and application thereof
  • Anticoagulant blackberry seed polysaccharides, and extraction separation method and application thereof
  • Anticoagulant blackberry seed polysaccharides, and extraction separation method and application thereof

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Embodiment Construction

[0026] The above-mentioned content of the present invention will be further described in detail below through specific embodiments.

[0027] Blackberry seeds were purchased from the blackberry planting base in Fengqiu County, Henan Province. Rubus spp. Blackberry seed.

[0028] The ethanol concentration in this article is the volume concentration.

[0029] 1. The extraction and separation method of anticoagulant blackberry seed polysaccharides includes the following steps: (1) Crush the blackberry seeds, degrease with petroleum ether for 3 times at room temperature, evaporate the solvent petroleum ether, and extract the dregs with 70% ethanol for 3 times , filter, dry the residue in the shade, then add distilled water to the residue at a ratio of 20mL / g to the mass-volume ratio of the material to liquid for extraction, extract 3 times at 80°C for 3 hours each time, suction filter while it is hot, combine the filtrates, concentrate under reduced pressure, and then add Add 95%...

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Abstract

The invention belongs to the technical field of plant extraction, and particularly relates to anticoagulant blackberry seed polysaccharides, and an extraction separation method and application thereof. The extraction separation method comprises the following steps: after pulverizing blackberry seeds, carrying out water extraction and alcohol precipitation to obtain crude polysaccharides, and respectively carrying out DEAE-52 cellulose column chromatography and SephadexG-100 gel column chromatography to obtain the three polysaccharides. The special extraction separation method is adopted to separate the three polysaccharides from the blackberry seeds; and the inspection on the in-vitro coagulation effect of the three polysaccharides proves that the three polysaccharides have favorable anticoagulant effect.

Description

technical field [0001] The invention belongs to the technical field of plant extraction, and in particular relates to an anticoagulant blackberry seed polysaccharide and its extraction and separation method and application. Background technique [0002] blackberries Rubus spp.Blackberry is an aggregate fruit plant of Rubus genus Rosaceae. It is not used for medicine. It is one of the four emerging small fruit trees in the world. It is native to North America. Its fruit is sweet and sour, with high nutritional value and Medicinal value, in addition to eating fresh, most of them are made into foods such as quick-frozen fruits, fruit juices, fruit wines and jams. Literature search found that research on blackberry mainly focused on fruit volatile oil, fatty acid composition of blackberry seed oil, and content analysis of flavonoids, anthocyanins and vitamin E in blackberry leaves and seeds. [0003] Blackberry seeds are by-products of processed products such as blackberry win...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00A61K31/715A61P7/02
Inventor 康文艺魏金凤张伟连朋丽谢平耀
Owner HENAN UNIVERSITY
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