Application of berberine in preparation of medicine for treating acute myeloid leukemia
A technology for acute myeloid and leukemia, applied in the field of medicine, can solve problems such as the research gap of acute myeloid leukemia, and achieve the effects of low cost, small side effects and good apoptosis rate
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Embodiment 1
[0024] Example 1. Morphological effects of berberine on the standard cell line MV-41 of refractory acute myeloid leukemia
[0025] The experimental materials used in this example are as follows: MV-41 cell line (refractory acute myeloid leukemia cells) was purchased from ATCC in the United States; Berberine (Berberine, content 98.5%) was purchased from Shaanxi Dadi Pharmaceutical Company, and the production batch number is 20111016; Cytarabine was purchased from Pfizer Pharmaceutical Co., Ltd.; DMEM medium was purchased from GIBCO; fetal bovine serum was purchased from Zhejiang Tianhang Biotechnology Co., Ltd.; NaHCO3 was purchased from Shanghai Jiuyi Chemical Reagent Co., Ltd., Cat.No.11810 -033; trypsin, EDTA, penicillin G sodium salt, and streptomycin sulfate were purchased from AMRESCO; absolute ethanol was purchased from Chongqing Chuandong Chemical Co., Ltd.; PBS was purchased from Beijing Zhongshan Jinqiao Biotechnology Co., Ltd.; From German Leica company, the model is...
Embodiment 2
[0030] Embodiment 2, berberine to refractory acute myeloid leukemia standard cell line MV-41 half inhibitory concentration
[0031] The experimental materials used in this example are as follows: MTT (tetramethylazolazolium blue) reagent was purchased from Sigma Company; enzyme-linked immunoassay instrument was purchased from Bio-RAD Company. MV-41 cell line at 37°C, 5% CO 2 Subculture was carried out in an incubator with saturated humidity, and the cells were used for experiments when they were in the logarithmic growth phase.
[0032] Experimental method: prepare a single cell suspension with culture medium containing 10% fetal bovine serum, and use 2×10 per well 5 / mL inoculated in a 96-well culture plate with a volume of 100 μL per well, and then moved the culture plate into CO 2 in an incubator at 37 °C, 5% CO 2 and cultured for 24 h under saturated humidity conditions. Remove the culture medium, then add berberine 0.9% sodium chloride aqueous solution and cytarabine ...
Embodiment 3
[0038] Example 3. Verification of the proliferative activity of berberine on the standard cell line MV-41 of refractory acute myeloid leukemia
[0039] According to Example 2, on the basis of preliminarily determining the half-maximum inhibitory concentration and time of berberine to MV-41, the selected concentration is 50 μg / ml, 100 μg / ml, 150 μg / ml and 200 μg / ml of berberine and the concentration is 100 μg / ml, 200 μg / ml, 400μg / ml Cytarabine acted on MV-41 cells for 24h, 48h and 72h respectively, and the inhibition rate was detected. The results are shown in Table 2.
[0040] Table 2, the inhibition rate (%) n=3 of different berberine effects on MV-41 cells at different times
[0041]
[0042] As can be seen from Table 2, as the concentration of berberine increased, its inhibitory rate to cells also increased significantly. When the berberine concentration was 50 μg / ml, the 24h inhibition rate was 61.61%; when the concentration increased to 200 μg / ml , the inhibition rat...
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