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Bacillus subtilis culture medium and preparation method thereof

A technology of Bacillus subtilis and culture medium, applied in the field of culture medium, can solve the problems of low activity of Bacillus subtilis, low spore yield, low stability, etc., and achieve the effects of low cost, high spore yield and good stability

Active Publication Date: 2015-11-04
广东中山市茂辉生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The object of the present invention is to provide a kind of Bacillus subtilis culture medium and preparation method thereof, thus solve the problems of low spore yield, high cost, low activity of Bacillus subtilis and low stability

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Formula: 4g sweet potato, 7g peptone, 0.05g ammonium nitrate, 0.4g potassium hydroxide, 0.5g calcium hydrogen phosphate, 0.3g magnesium bicarbonate, 8g wolfberry, 6g sugarcane, 7g chestnut, 5g trichosanthes, 7g Cynomorium, o-hydroxy Sodium benzoate 0.05g, 8-hydroxyquinoline 0.01g and deionized water 150g.

[0023] Preparation Process:

[0024] The first step is to take sweet potato, wolfberry and Cynomorium, add deionized water, soak for 1 hour, then boil for 20 minutes, filter, and take the filtrate for later use;

[0025] In the second step, the sugarcane, trichosanthemi, and chestnut are mixed, ground, and filtered to 0.2um to obtain a fine powder, and the fine powder filtration rate reaches 100%;

[0026] The third step is to cool the filtrate obtained in the first step to a temperature of 80°C, mix the fine powder obtained in the second step with other remaining raw materials according to the proportion, stir the mixture uniformly for 30 minutes, and use NaOH with...

Embodiment 2

[0028] Formula: 8g sweet potato, 13g peptone, 0.3g ammonium nitrate, 0.8g potassium hydroxide, 1g calcium hydrogen phosphate, 0.6g magnesium bicarbonate, 12g wolfberry, 13g sugarcane, 10g chestnut, 8g trichosanthes, Cynomorium 10g, o-hydroxybenzene Sodium formate 0.2g, 8-hydroxyquinoline 0.3g and deionized water 200g.

[0029] Preparation Process:

[0030] The first step is to take sweet potato, wolfberry and Cynomorium, add deionized water, soak for 2 hours, then boil for 30 minutes, filter, and take the filtrate for later use;

[0031] In the second step, the sugarcane, trichosanthemi, and chestnut are mixed, ground, and filtered to 0.22um to obtain a fine powder, and the fine powder filtration rate reaches 100%;

[0032] The third step is to cool the filtrate obtained in the first step to a temperature of 90°C, mix the fine powder obtained in the second step with other remaining raw materials according to the ratio, and stir the mixture uniformly for 40 minutes, and the co...

Embodiment 3

[0034] Recipe: 5g sweet potato, 8g peptone, 0.1g ammonium nitrate, 0.4g potassium hydroxide, 0.5g calcium hydrogen phosphate, 0.3g magnesium bicarbonate, 9g wolfberry, 6g sugarcane, 7g chestnut, 5g trichosanthes, cynomorium 8g, o-hydroxy Sodium benzoate 0.1g, 8-hydroxyquinoline 0.1g and deionized water 150g.

[0035] Preparation Process:

[0036] The first step is to take sweet potato, wolfberry and Cynomorium according to the ratio, add deionized water, soak for 2 hours, then boil for 30 minutes, filter, and take the filtrate for later use;

[0037] In the second step, the sugarcane, trichosanthemi, and chestnut are mixed, ground, and filtered to 0.21um to obtain fine powder, and the fine powder filtration rate reaches 100%;

[0038]The third step is to cool the filtrate obtained in the first step to a temperature of 85°C, mix the fine powder obtained in the second step with other remaining raw materials according to the ratio, stir the mixture uniformly for 35 minutes, and ...

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Abstract

The invention discloses a Bacillus subtilis culture medium and a preparation method thereof. The culture medium is prepared from components in parts by weight as follows: 4-8 parts of sweet potatoes, 7-13 parts of peptone, 0.05-0.3 parts of ammonium nitrate, 0.4-0.8 parts of potassium hydroxide, 0.5-1 part of calcium hydrophosphate, 0.3-0.6 parts of magnesium bicarbonate, 8-12 parts of Chinese wolfberries, 6-13 parts of sugarcane, 7-10 parts of seguin chestnuts, 5-8 parts of snakegourd roots, 0.05-0.2 parts of sodium salicylate, 0.01-0.3 parts of 8-hydroxyquinoline and 70-90 parts of deionized water. The Bacillus subtilis culture medium and the preparation method thereof have the benefits as follows: the culture medium is applied to culture of Bacillus subtilis, the spore yield is high, the cost is low, and the obtained Bacillus subtilis has the higher anti-bacterial activity and the good stability.

Description

technical field [0001] The invention relates to the field of culture medium, in particular to a culture medium for Bacillus subtilis and a preparation method thereof. Background technique [0002] Bacillus subtilis is a species of Bacillus. A single cell is 0.7~0.8×2~3 microns, evenly colored. Uncapsulated, with periosteum flagella, able to move. Gram-positive bacteria, spores 0.6-0.9×1.0-1.5 microns, oval to columnar, located in the center of the bacterium or slightly biased, the bacterium does not expand after spore formation. The surface of the colony is rough and opaque, stained white or yellowish, and often forms wrinkles when growing in liquid medium. Aerobic bacteria. It can use protein, various sugars and starch to decompose tryptophan to form indole. It is widely used in genetic research, and the synthesis pathway and regulation mechanism of purine nucleotides in this bacterium are relatively clear. Widely distributed in soil and decaying organic matter, it ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12R1/125
Inventor 马艳辉张磊周爱凤
Owner 广东中山市茂辉生物科技有限公司
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