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A strain of Bacillus amyloliquefaciens and its application in the control of rice false smut

A technology for dissolving starch spores and rice false smut in rice is applied in the fields of application, bacteria, fungicides, etc. It can solve the problems of long period of disease-resistant varieties, single resistance, pesticide residues, etc., and achieve good application prospects, fast growth, The effect of large spore production

Active Publication Date: 2018-06-29
CHINA KINGDOM AGRITECH QINGDAO
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The disease-resistant variety has a long cycle and a single resistance
The use of chemicals for chemical control takes a long time and a large amount of use, resulting in pesticide residues and environmental pollution; at the same time, the long-term use of chemical pesticides makes pathogenic bacteria resistant to them, resulting in decreased control efficiency or even failure

Method used

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  • A strain of Bacillus amyloliquefaciens and its application in the control of rice false smut
  • A strain of Bacillus amyloliquefaciens and its application in the control of rice false smut
  • A strain of Bacillus amyloliquefaciens and its application in the control of rice false smut

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] 1. Isolation and purification of Bacillus amyloliquefaciens strain QZ-003H

[0028] Bacillus amyloliquefaciens bacterial strain QZ-003H of the present invention is to adopt dilution plate method and plate streaking method to separate and obtain from soil, and separation method is:

[0029] ⑴Isolation of Bacillus: Soil sample collection, select plots planted with different types of crops in different regions of the country, use 5-point sampling method, collect appropriate amount of soil around and in the center of the plot within a depth of 10-20cm, and the same amount Mix well. Indicate the location, time and person of the collection. Weigh 1g of soil sample into 100mL of sterile water, place it in a shaker at 30°C at 150rpm for 10min, then place it in a water bath at 60°C for 30min, take 100μL of 10 -2 、10 -3 、10 -4 The diluted solution is spread on the beef extract peptone medium plate, and each gradient is coated in three parallels. After culturing at 30°C for 2 ...

Embodiment 2

[0040] 1. Fermentation process of Bacillus strain QZ-003H

[0041] Beef extract peptone liquid medium, the formula is tryptone 10g, beef extract 3g, sodium chloride 5g, distilled water 1000mL, pH adjusted to 7.0-7.2.

[0042] A large amount of solid fermentation medium formula (mass percentage): solid material: corn cob 60%, bran 40%; the components and mass percentage of the inorganic salt solution are: potassium dihydrogen phosphate 3.5%, magnesium sulfate 0.04% , 4% ammonium sulfate, and the rest is water. The solid-to-liquid ratio is 1:1.8 (mass ratio).

[0043] Mass solid fermentation process of Bacillus amyloliquefaciens strain QZ-003H:

[0044] ① Bacteria seed liquid culture: Pick a small amount of Bacillus amyloliquefaciens strain QZ-003H from the inclined surface of the test tube, transfer it to beef extract peptone liquid medium, shake and culture at 30°C for 3-5 days, and this is the seed liquid;

[0045] ②Cultivation of solid production strains: Inoculate the se...

Embodiment 3

[0048] Embodiment 3 field control test

[0049] 1 Purpose of the test

[0050] The present invention conducts a field drug efficacy test of 1 billion spores / gram Bacillus amyloliquefaciens (QZ-003H) wettable powder for preventing and treating rice false smut, in order to determine the optimal field use amount, drug efficacy and effect of the agent for preventing and treating rice false smut Characteristics, duration of efficacy, effects of agents on crops and non-target beneficial organisms, and safe and reasonable practical technologies provide data.

[0051] 2 test conditions

[0052] 2.1 Selection of test objects, crops and varieties

[0053] Test object: rice smut disease ustilaginoldea virens;

[0054] Experimental crop: single season late rice, variety Ning 81.

[0055] 2.2 Crop cultivation and environmental conditions

[0056] The test field is located in Shangwanling Village, Qiuai Town, Yinzhou District, Zhejiang Province. The area of ​​rice in the test area is 1...

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Abstract

The invention discloses a bacillus amyloliquefaciens and its application in preventing and treating rice false smut. The bacterial strain is Bacillus amyloliquefaciens QZ-003H, and the preservation number is CGMCC No.11051. The invention also discloses a biological control preparation containing the above-mentioned Bacillus amyloliquefaciens strain QZ-003H and a preparation method thereof. Experiments show that the Bacillus amyloliquefaciens strain of the present invention has the characteristics of fast growth, large spore production, strong stress resistance, rapid and large-scale colonization in the rice rhizosphere, etc., and thus has good application prospects. The biological control preparation prepared by the bacillus amyloliquefaciens can be used as a biological pesticide or a biological fertilizer to prevent and treat rice false smut.

Description

technical field [0001] The invention belongs to the field of biological control of plant diseases. Specifically, the present invention relates to a bacillus capable of efficiently preventing and controlling plant diseases and its use, and a biological control agent prepared by using the bacillus. Background technique [0002] Plant diseases seriously affect the growth of agricultural and forestry crops, and even cause crop death, resulting in significant economic losses. For the prevention and control of plant diseases, conventional methods include traditional chemical control, breeding of disease-resistant varieties, and biological control. The disease-resistant variety has a long cycle and a single resistance. Chemical control uses a long time and a large amount of pesticides, resulting in pesticide residues and environmental pollution. At the same time, the long-term use of chemical pesticides makes pathogenic bacteria resistant to them, resulting in reduced control eff...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A01N63/00A01P3/00A01G13/00C12R1/07
Inventor 栾守业牛赡光牛兆颖刘幸红崔凤云
Owner CHINA KINGDOM AGRITECH QINGDAO
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