A kind of rapid propagation method of short-distance grove japonica
A kind of short-distance grove, fast technology, applied in the field of plant tissue culture, can solve the problems of low coefficient, difficult to meet the requirements of protection and development, difficult to multiply and so on, achieve simple preparation, solve the problem of resource protection, transplant survival rate high effect
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Embodiment 1
[0018] (1) Seed germination: Use a healthy, disease-free plant as the mother plant, and artificially pollinate the short-distance grooved plant according to the routine, that is, in May to June each year, that is, the flowering season of short-distance grooved plant. 5-7 days after flowering, select healthy and disease-free plants for pollination and self-pollination at 10-12 a.m. on a sunny and rain-free day. The plants are not sprayed with water one day before and one day after selfing; 120 days after pollination, The mature, disease-free, and unruptured intact fruit of the capsule is routinely sterilized, that is, the capsule of the short-distance groswellia is rinsed with running water for 30 minutes, and the surface of the capsule is wiped with 70% alcohol on the super bacteria workbench, and then sequentially Wash with sterile water 3 times, sterilize with 0.1% mercury liters for 10 minutes, wash 3 times with sterile water, then soak with 10% sodium hypochlorite for 10 min...
Embodiment 2
[0026] (1) Seed germination: Use a healthy, disease-free plant as the mother plant, and artificially pollinate the short-distance grooved plant according to the routine, that is, in May to June each year, that is, the flowering season of short-distance grooved plant. 5-7 days after flowering, select healthy and disease-free plants for pollination and self-pollination at 10-12 a.m. on a sunny and rain-free day. The plants are not sprayed with water one day before and one day after selfing; 120 days after pollination, The mature, disease-free, and unruptured intact fruit of the capsule is routinely sterilized, that is, the capsule of the short-distance groswellia is rinsed with running water for 30 minutes, and the surface of the capsule is wiped with 70% alcohol on the super bacteria workbench, and then sequentially Wash with sterile water 3 times, sterilize with 0.1% mercury liters for 10 minutes, wash 3 times with sterile water, then soak with 10% sodium hypochlorite for 10 min...
Embodiment 3
[0034] (1) Seed germination: Use a healthy, disease-free plant as the mother plant, and artificially pollinate the short-distance grooved plant according to the routine, that is, in May to June each year, that is, the flowering season of short-distance grooved plant. 5-7 days after flowering, select healthy and disease-free plants for pollination and self-pollination at 10-12 a.m. on a sunny and rain-free day. The plants are not sprayed with water one day before and one day after selfing; 120 days after pollination, The mature, disease-free, and unruptured intact fruit of the capsule is routinely sterilized, that is, the capsule of the short-distance groswellia is rinsed with running water for 30 minutes, and the surface of the capsule is wiped with 70% alcohol on the super bacteria workbench, and then sequentially Wash with sterile water 3 times, sterilize with 0.1% mercury liters for 10 minutes, wash 3 times with sterile water, then soak with 10% sodium hypochlorite for 10 min...
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