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Photosensitizer nanoparticle as well as preparation method and application thereof

A photosensitizer and nanoparticle technology, applied in the field of biomedicine, can solve the problems of unclear components, low drug loading, large amount of excipients, etc., and achieve the effects of improving enrichment, high drug loading, and mild experimental conditions.

Active Publication Date: 2015-10-21
INST OF PROCESS ENG CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Because its preparation method is that epirubicin and chlorin are included in lecithin by thin film dispersion method-pH gradient method to form complex liposomes, so that epirubicin complex liposomes can better target To tumor tissue, it still has defects such as low drug loading, large amount of excipients, and unclear components.

Method used

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  • Photosensitizer nanoparticle as well as preparation method and application thereof
  • Photosensitizer nanoparticle as well as preparation method and application thereof
  • Photosensitizer nanoparticle as well as preparation method and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0053] (1) Configure the aqueous solution of chlorin e6 with a volume mass concentration of 1 mg / mL, because of its poor water solubility, use alkali to adjust the pH value of the solution to 10-11 to increase its solubility, without adding an organic solvent; configure An aqueous solution of fluorenylmethoxycarbonyl-L-lysine hydrochloride with a volume mass concentration of 4 mg / mL.

[0054] (2) Add 1 mL of the aqueous solution of the above chlorin e6 to 1 mL of the aqueous solution of fluorenylmethoxycarbonyl-L-lysine hydrochloride, and mix well.

[0055] (3) Adjust the pH of the mixed solution to 5-7 to obtain photosensitizer nanoparticles containing chlorin e6.

[0056] The particle size distribution of photosensitizer nanoparticles obtained in this embodiment is as follows: Figure 1a shown; its transmission electron microscope image is shown in figure 2 shown; its enzyme-responsive release is as follows Figure 6 shown. From Figure 1a , figure 2 as well as Fig...

Embodiment 2

[0058] (1) configuration volume mass concentration is the aqueous solution of verteporfin of 1mg / mL, because of its poor water solubility, adjust solution pH value to 10-11 to increase solubility, do not need to add organic solvent; 4mg / mL aqueous solution of L-phenylalanine-L-phenylalanine amide, because of its poor water solubility, adjust the pH value to 2-3 to increase solubility.

[0059] (2) Add 1 mL of the above verteporfin aqueous solution to 1 mL of the L-phenylalanine-L-phenylalanine amide aqueous solution, and mix well.

[0060] (3) Adjusting the pH of the mixed solution to 5-7 to obtain photosensitizer nanoparticles containing verteporfin.

[0061] The particle size distribution of photosensitizer nanoparticles obtained in this embodiment is as follows: Figure 1b shown; its transmission electron microscope image is shown in image 3 shown, from Figure 1b and image 3 It can be seen that the self-assembly of verteporfin is regulated by L-phenylalanine-L-phenyl...

Embodiment 3

[0063] (1) Configure an aqueous solution of chlorin e6 with a volume concentration of 1 mg / mL. Because of its poor water solubility, add 0.1M NaOH to it to help dissolve, adjust the pH value of the solution to 10-11, and do not need to add organic solvents ; Configure the aqueous solution of doxorubicin hydrochloride whose volume mass concentration is 4mg / mL.

[0064] (2) 1 mL of the above-mentioned aqueous solution of chlorin e6 was added to 1 mL of an aqueous solution of doxorubicin hydrochloride.

[0065] (3) Adjusting the pH of the mixed solution to 5.0-7.0 to obtain photosensitizer nanoparticles containing chlorin e6 and doxorubicin.

[0066] The particle size distribution of photosensitizer nanoparticles obtained in this embodiment is as follows: Figure 1c shown; its transmission electron microscope image is shown in Figure 4 shown, from Figure 1c and Figure 4 It can be seen that the self-assembly of chlorin e6 is regulated by doxorubicin hydrochloride to form ph...

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Abstract

The invention relates to a photosensitizer nanoparticle as well as a preparation method and application thereof. The photosensitizer nanoparticle comprises components of photosensitizer molecules and regulation and control molecules for conducting regulation and control on the composition structure of the photosensitizer; the preparation of the photosensitizer nanoparticle is realized through self-composition of the photosensitizer molecules and the regulation and control molecules. The photosensitizer nanoparticle has the advantages that the size is controllable, the distribution is uniform, and the medicine loading capacity are high; the preparation method is simple and quick, and does not need to use any toxic solvent or additive; the photosensitizer nanoparticle can be applied to preparation of medicines for tumor positioning image formation and photodynamic therapy, has the characteristics of being high in biocompatibility and high in tumor tissue gathering, and has excellent application prospects when being used as the anti-tumor and photodynamic therapy medicine.

Description

technical field [0001] The present invention relates to the technical field of biomedicine, in particular to a photosensitizer nanoparticle and its preparation method and application, in particular to a photosensitizer nanoparticle based on molecular assembly and its preparation method and their use in the preparation of tumor imaging drugs and photodynamic therapy drugs the use of. Background technique [0002] Photodynamic therapy is clinically used to treat many diseases, including tumors, especially superficial tumors, such as esophageal tumors, bladder tumors, and melanoma. Photodynamic therapy is characterized by high spatiotemporal selectivity and low systemic damage. Photosensitizers play an important role in photodynamic therapy. After absorbing light of a specific wavelength, photosensitizers become excited molecules, which react with oxygen to generate singlet oxygen, which can kill tumor cells. Although photosensitizer molecules are constantly developing, they ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K41/00A61K9/51A61K49/00A61P35/00
Inventor 闫学海邹千里刘凯
Owner INST OF PROCESS ENG CHINESE ACAD OF SCI
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