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Oleanolic acid liposome coated with nanogold spherical shell and preparation method thereof

A technology of oleanolic acid lipids and nano-liposomes, which is applied in the direction of liposome delivery, medical preparations with non-active ingredients, medical preparations containing active ingredients, etc., which can solve the problems of poor targeting, capture, Poor stability and other problems, to achieve the effect of simple preparation method, enhanced stability and high repeatability

Active Publication Date: 2015-10-21
YANSHAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, conventional nanoliposomes have a series of defects, such as being easily captured by the phagocytic system of monocytes, poor targeting and poor stability, etc.

Method used

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  • Oleanolic acid liposome coated with nanogold spherical shell and preparation method thereof
  • Oleanolic acid liposome coated with nanogold spherical shell and preparation method thereof
  • Oleanolic acid liposome coated with nanogold spherical shell and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] 45mg soybean lecithin (purchased from Shenyang Tianfeng Biopharmaceutical Co., Ltd.), 4.0mg cholesterol (purchased from Tianjin Damao Chemical Instrument Supply Station) and 5.0mg oleanolic acid (purchased from Sichuan Shifang Huakang Pharmaceutical Raw Material Factory ), dissolved in 3mL of absolute ethanol, and obtained a homogeneous oil phase, i.e. the organic phase, by magnetic stirring. Under magnetic stirring, 3 mL of the organic phase was slowly and uniformly dropped into 10 mL of PBS buffer solution at 37° C. and pH 6.0 to obtain a liposome suspension. Place on a shaker at 24° C., 100 rpm, and remove absolute ethanol in the liposome suspension for 30 minutes to obtain oleanolic acid nanoliposomes.

[0027] Dissolve 7 mg of chitosan in 1 mL of 0.05 M glacial acetic acid solution to obtain a chitosan solution. Under the stirring of magnetic stirrer, chitosan solution is added dropwise in liposome, and the volume ratio of chitosan and oleanolic acid nano liposome...

Embodiment 2

[0032] 55mg soybean lecithin (purchased from Shenyang Tianfeng Biopharmaceutical Co., Ltd.), 5.5mg cholesterol (purchased from Tianjin Damao Chemical Instrument Supply Station) and 5mg oleanolic acid (purchased from Sichuan Shifang Huakang Pharmaceutical Raw Material Factory) , was dissolved in 5 mL of absolute ethanol, and a uniform oil phase, ie, an organic phase, was obtained by magnetic stirring. Under magnetic stirring, 5 mL of the organic phase was slowly and uniformly dropped into 10 mL of PBS buffer solution at 43° C. and pH 6.8 to obtain a liposome suspension. Place on a shaker at 26° C., 115 rpm, and remove the absolute ethanol in the liposome suspension for 45 minutes to obtain oleanolic acid nanoliposomes.

[0033] Dissolve 10 mg of chitosan in 1 mL of 0.10 M glacial acetic acid solution to obtain a chitosan solution. Under the stirring of magnetic stirrer, chitosan solution is added dropwise in liposome, and the volume ratio of chitosan and oleanolic acid nano li...

Embodiment 3

[0038] 60mg soybean lecithin (purchased from Shenyang Tianfeng Biopharmaceutical Co., Ltd.), 7.0mg cholesterol (purchased from Tianjin Damao Chemical Instrument Supply Station) and 5mg oleanolic acid (purchased from Sichuan Shifang Huakang Pharmaceutical Raw Material Factory) , was dissolved in 6 mL of absolute ethanol, and a uniform oil phase, ie, an organic phase, was obtained by magnetic stirring. Under magnetic stirring, 6 mL of the organic phase was slowly and uniformly dropped into 10 mL of PBS buffer solution at 48° C. and pH 7.4 to obtain a liposome suspension. Place on a shaker at 29° C., 130 rpm, and remove absolute ethanol in the liposome suspension for 60 minutes to obtain oleanolic acid nanoliposomes.

[0039] Dissolve 13 mg of chitosan in 1 mL of 0.15 M glacial acetic acid solution to obtain a chitosan solution. Under the stirring of magnetic stirrer, chitosan solution is added dropwise in liposome, and the volume ratio of chitosan and oleanolic acid nano liposo...

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Abstract

Provided is an oleanolic acid liposome coated with a nanogold spherical shell. According to the oleanolic acid liposome coated with the nanogold spherical shell, the surface of the oleanolic acid nano liposome is coated with a compact shell composed of gold nanoparticles, and the compact shell and the liposome constitute a nanosphere with the particle size 100-200 nm; a preparation method of the oleanolic acid liposome coated with the nanogold spherical shell comprises the steps that chitosan serves as an intermediate medium of the liposome and the gold nanoparticles and is used for modifying the liposome to obtain the nanoliposome modified by the chitosan; incubation is conducted on the liposome and gold nanoparticles obtained through reduction with sodium borohydride in a particular situation, then a gold chloride solution with a certain concentration is added, and finally the incubation is continuously conducted for a period of time by adding an ascorbic acid solution. According to the oleanolic acid liposome coated with the nanogold spherical shell and the preparation method thereof, the preparation can be completed under mild conditions, the preparation method is simple, and reaction is easy to control; according to the prepared oleanolic acid liposome coated with the nanogold spherical shell, the near-infrared absorption exists between 650 nm and 900 nm, and damage to human normal tissues is reduced.

Description

[0001] Technical Field The present invention relates to a nanoliposome for treating cancer and a preparation method thereof. Background technique [0002] In today's society, cancer has become the second killer of human health, and the cure of cancer has always been a major problem in the world. Currently, the most common methods of treating cancer include chemotherapy, radiotherapy and surgery. However, these current treatment methods all cause great toxic side damage to the body, and malignant tumors are difficult to be cured, and the best treatment opportunities are often missed due to untimely detection. Therefore, the urgent problem to be solved at present is to find a new therapeutic method that can eliminate tumor cells without harming normal cells. [0003] In recent years, drug carriers have received extensive attention. Liposome is a lipid self-assembled structure in aqueous solution, which is a biocompatible and promising drug carrier. Liposomes generally improve...

Claims

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Application Information

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IPC IPC(8): A61K41/00A61K9/127A61K9/51A61K47/24A61K47/28A61K47/36A61K31/56A61P35/00
Inventor 高大威边艳红刘艳平张旭武李楠籍冰朔
Owner YANSHAN UNIV
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