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Biosensor for detecting hydrogen peroxide and polyphenol compounds at high sensitivity, and preparation and application thereof

A polyphenolic compound and biosensor technology, applied in the field of bioanalysis, can solve the problems of complex sensor preparation process and low sensitivity, and achieve the effect of promoting electron transfer, simple process and good repeatability

Inactive Publication Date: 2015-10-14
NANJING UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] In view of the complex preparation process and low sensitivity of the prior art sensor, our purpose is to provide a biosensor, its preparation and its application

Method used

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  • Biosensor for detecting hydrogen peroxide and polyphenol compounds at high sensitivity, and preparation and application thereof
  • Biosensor for detecting hydrogen peroxide and polyphenol compounds at high sensitivity, and preparation and application thereof
  • Biosensor for detecting hydrogen peroxide and polyphenol compounds at high sensitivity, and preparation and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Example 1: Using a glassy carbon electrode with a diameter of 3 mm, the electrode is polished to a mirror surface in a 0.05 μm alumina suspension, and then ultrasonicated in ethanol and water for 2 minutes. After drying with nitrogen, the electrode is placed in a solution containing 10 mmol / L In EDOT and 0.3 mg / ml PSS aqueous solution, EDOT is polymerized on the electrode by cyclic voltammetry, the scanning potential of cyclic voltammetry is -0.2 ~ 0.8V, the scanning rate is 0.05V / s, and the number of polymerization cycles is 6 lock up.

Embodiment 2

[0034] Example 2: Same as Example 1, the pre-treatment process of the electrode remains unchanged, only the scanning potential of EDOT polymerized by cyclic voltammetry is changed, the scanning potential is set to 0-1.2V, and the scanning rate is 0.15V / s. The polymerization circle The number is 10 circles.

Embodiment 3

[0035] Embodiment 3: same as embodiment 1, the pre-treatment process of the electrode remains unchanged, the modification of the PEDOT film only changes the scanning potential of cyclic voltammetry polymerization EDOT, the scanning potential is set to be -0.1~1.0V, and the scanning rate is 0.1V / s , the number of polymerization circles is 8 circles.

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Abstract

The invention discloses a biosensor for detecting hydrogen peroxide and polyphenol compounds at high sensitivity, and preparation and application thereof. The biosensor is a biomimetic sensor, which is formed from 1-pyrenebutyric acid and hemachrome through the coordination chemical effect of zirconium ions, and is characterized by connecting PEDOT (Polymer Ethylenedioxy Thiophene) with 1-pyrenebutyric acid through phi-phi conjugation, wherein the hemachrome is taken as a main body, PEDOT is taken as a decorative material and 1-pyrenebutyric acid is taken as an intermediate connection agent. PEDOT is a conductive macromolecule capable of greatly promoting electron transfer, so that the detection sensitivity of the sensor is increased; 1-pyrenebutyric acid and PEDOT are connected through phi-phi conjugation, the phi-phi conjugation acting force is very strong, and 1-pyrenebutyric acid can be stably connected onto PEDOT; the hemachrome is connected onto 1-pyrenebutyric acid through coordination formed by zirconium ions and carboxyl, the conjugation effect of zirconium ions is easy to form, and more hemachrome can be combined on the surface of an electrode of same area; and the whole biomimetic sensor is mainly prepared by polymerizing and immersing, and the manner is simple to operate and good in repeatability.

Description

technical field [0001] The invention belongs to the technical field of bioanalysis, and in particular relates to a biosensor, its preparation and its application, in particular to a biosensor for highly sensitive detection of hydrogen peroxide and polyphenol compounds, its preparation and its application. Background technique [0002] Porphyrin and its derivative compounds widely exist in important organelles related to energy transfer in organisms. Iron porphyrin (Hemin) is the active center of hemoglobin and plays an important role in the process of transporting oxygen. Hemoglobin has a similar structure to iron porphyrin, and ferric ion is its active center. Hemoglobin has extremely high catalytic performance for nitrogen, oxygen, nitric oxide, carbon dioxide and peroxide. The ferric ions at the position are provided in the process of gaining and losing electrons. However, due to the lack of a suitable biomimetic environment, its electron transfer efficiency is much low...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/30G01N27/26B82Y15/00
Inventor 孔金明于雪花胡琼胡伟文张学记
Owner NANJING UNIV OF SCI & TECH
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