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Sedum rhizosphere lead-resistant strain pseudomonas as well as screening method and application thereof

A technology of Pseudomonas and strains, which is applied in the screening and application fields of Sedum rhizosphere lead-resistant strain Pseudomonas, and can solve the problems of human health hazards, soil pollution, and damage to the normal function of the ecosystem.

Inactive Publication Date: 2015-10-14
CHINA THREE GORGES CORPORATION +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the average global discharge of lead is about 5 million tons per day, a considerable part of which enters the soil, which pollutes the soil in some areas, destroys the normal function of the ecosystem, and also causes harm to human health.

Method used

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  • Sedum rhizosphere lead-resistant strain pseudomonas as well as screening method and application thereof
  • Sedum rhizosphere lead-resistant strain pseudomonas as well as screening method and application thereof
  • Sedum rhizosphere lead-resistant strain pseudomonas as well as screening method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Example 1 Sedum rhizosphere lead-resistant strain Pseudomonas sp.JPb5 isolation

[0029] Materials and Methods

[0030] 1. Medium

[0031] Bacterial culture medium: beef extract 3g, peptone 10g, NaCl 5g, distilled water 1000mL, pH7.0.

[0032] Prepare the screening medium: the basic medium is 1000ml of bacterial culture medium, add 10mL of trace element solution and vitamin solution, sterilize at 121°C for 20min, cool to about 70°C, add lead solution mother liquor, so that the lead content in the medium is 1000mg· L -1 . Incubate at 37°C.

[0033] The trace element solution is: MnSO 4 0.01g, ZnSO 4 0.05g, H 3 BO 3 0.01g, CaCl 2 0.01g, dilute to 1L, and store at 4°C in the dark.

[0034] The vitamin solution is: creatine 0.025g, ascorbic acid 0.025g, riboflavin 0.025g, citric acid 0.02g, dilute to 1L, and store in the dark at 4°C.

[0035] Lead solution mother liquor: Pb(CH 3 COOH) 2 ·3H 2 O formulated as Pb 2+ The concentration is 200mg·mL -1 The mother ...

Embodiment 2

[0038] Example 2 Identification of bacterial strain JPb5

[0039] 1. Genomic DNA extraction

[0040] The above-mentioned strain JPb5 was mass-cultured according to conventional technical means, and then its genomic DNA was obtained.

[0041] 2. Identification method of 16S rDNA of strain JPb5

[0042] 2.1 PCR amplification, sequencing and phylogenetic tree construction of 16S rDNA of strain JPb5

[0043] 2.1.1 PCR amplification of 16S rDNA gene sequence

[0044] The primers at both ends of the amplified 16S rDNA gene sequence are universal primers: forward primer BSF8 / 20: 5'-AGAGTTTGATCCTGGCTCAG-3' (SEQ ID NO: 1) and reverse primer BSR1541 / 20: 5'-AAGGAGGTGATCCAGCCGCA-3' (SEQ ID NO: 2). The PCR reaction system was 50 μL, and the reaction conditions were denaturation at 94°C for 5 minutes; followed by 35 cycle reactions: denaturation at 94°C for 45 s, annealing at 50°C for 45 s, and extension at 72°C for 90 s; then extension at 72°C for 10 min, and finally stored at 4°C.

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Abstract

The invention relates to sedum rhizosphere lead-resistant strain pseudomonas sp JPb5, in particular to a lead-resistant strain pseudomonas sp JPb5 separated from sedum rhizosphere. The lead-resistant strain pseudomonas sp JPb5 is preserved in the China General Microbiological Culture Collection Center with the preservation number of CGMCC No.10087 on December 1, 2014. The lead-resistant strain pseudomonas sp JPb5 is gram-negative bacteria or aerobasilus, and has flagella and capsules. The strain has relatively high resistance to heavy metal lead, and has practical significance and important engineering application value on the aspect of remediation of heavy metal lead contaminated soil through combination of plants and microorganisms.

Description

technical field [0001] The invention relates to a lead-resistant bacterial strain Pseudomonas sp. JPb5 isolated from the rhizosphere of Sedum sedum and its application in plant-microorganism combined restoration of lead-heavy metal-contaminated soil. Background technique [0002] Soil is the material basis for human beings to obtain food and other renewable resources, and it is the natural environment on which human beings depend and an important resource for agricultural production. [0003] The discharge of environmental pollutants is increasing day by day, environmental pollution and ecological damage have brought serious pollution to the soil, and the area polluted by heavy metals is increasing, which not only degrades soil fertility, reduces the yield and quality of agricultural products, but also deteriorates the water environment and It endangers human life and health through the food chain. According to rough statistics, the lead discharged into the environment glob...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20B09C1/10
CPCB09C1/10B09C1/105C12N1/20C12N1/205C12R2001/38
Inventor 林海龙范阳冯晶石田宇哲李德斌
Owner CHINA THREE GORGES CORPORATION
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