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Method of separation and extraction of D-lactic acid from sodium D-lactate fermentation liquid

A technology for lactic acid fermentation and fermentation broth, applied in the field of bioengineering, can solve the problems of low degree of automation in the separation process, low product quality and high energy consumption, and achieve the target of reducing nitrogen content and post-decolorization costs, reducing energy consumption and acidification. short time effect

Active Publication Date: 2015-10-14
SINOPEC YANGZI PETROCHEM +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The process is mature and easy to control, but there are disadvantages such as long process route, many unit operations, serious waste liquid pollution, high energy consumption, and low degree of automation of the whole separation process. The recovery rate of lactic acid is generally between 40% and 50%. The product obtained is of lower quality

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] The D-lactic acid fermentation broth of the Bacillus production strain, wherein the concentration of sodium D-lactate is 100g / L, has no residual sugar, and the pH of the fermentation broth is 6.0. Take the fermented D-sodium lactate fermentation broth and separate the bacterial cells through a disc centrifuge; then perform microfiltration and ultrafiltration to separate macromolecular proteins and some pigments. The molecular weight cut-off of the ultrafiltration membrane is 1000; For decolorization, powder activated carbon is used for decolorization, the decolorization pH is neutral, the decolorization temperature is 60°C, and the decolorization time is 30 minutes; the decolorized fermentation broth is sent to an ion exchange column for ion exchange, and the fermentation broth is first passed through a cation exchange resin. Anion exchange resin; finally, the fermented broth after ion exchange is sent to the evaporator for vacuum evaporation to obtain D-lactic acid with...

Embodiment 2

[0030] The D-lactic acid fermentation liquid of the strain produced by Bacillus, wherein the concentration of sodium D-lactate is 120g / L, has no residual sugar, and the pH of the fermentation liquid is 5.8. Take the D-sodium lactate fermentation broth after fermentation and directly enter the microfiltration and ultrafiltration system to separate macromolecular proteins and some pigments. The molecular weight cut-off of the ultrafiltration membrane is 5000; the fermentation broth is decolorized, and the decolorization is carried out by nanofiltration membrane. , the molecular weight cut-off used by the nanofiltration membrane is 300; the decolorized fermentation broth is sent to an ion exchange column for ion exchange, and the fermentation broth first passes through a cation exchange resin and then an anion exchange resin; finally, the ion-exchanged fermentation broth is sent to an evaporation column. D-lactic acid with an optical purity of 99% and a content of 91% was obtained...

Embodiment 3

[0032] The D-lactic acid fermentation broth of the Bacillus production strain, wherein the concentration of sodium D-lactate is 110g / L, has no residual sugar, and the pH of the fermentation broth is 6.0. The fermented D-sodium lactate fermentation broth is taken directly into the microfiltration and ultrafiltration system to separate macromolecular proteins and some pigments. The molecular weight cut-off of the ultrafiltration membrane is 20,000; the fermentation broth is decolorized, and the decolorization is carried out in an activated carbon column. Granular activated carbon is selected, the decolorization pH is 6.0, the decolorization temperature is 60°C, and the decolorization time is 30 minutes; the decolorized fermentation broth is sent to an ion exchange column for ion exchange, and the fermentation broth is first passed through a cation exchange resin and then an anion exchange resin; The fermented broth after ion exchange is sent to an evaporator for vacuum evaporatio...

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Abstract

The invention discloses a method of separation and extraction of D-lactic acid from sodium D-lactate fermentation liquid, belongs to the technical field of bio-engineering and particularly relates to the method of separation and extraction of D-lactic acid from the fermentation liquid. The method includes the steps of (1) performing solid-liquid separation to the sodium D-lactate fermentation liquid, removing proteins and pigments to obtain a clarified fermentation liquid; (2) decoloring the clarified fermentation liquid obtained in the step (1); (3) performing ion exchange to the fermentation liquid obtained in the step (2); and (4) concentrating the fermentation liquid after the ion exchange to obtain the D-lactic acid. In the method, only is normal-temperature acidification required so that the method is short in acidification time. The method is effectively reduced in the content of protein and a part of the pigments in the fermentation liquid and is free of generation of calcium sulfate which is low in additional value and is difficult to treat. The method is optimized in operation and is less in separation steps.

Description

technical field [0001] The invention belongs to the technical field of bioengineering, and in particular relates to a method for separating and purifying D-lactic acid in fermentation broth. Background technique [0002] Lactic acid is one of the three major organic acids in the world, it can be widely used in food, medicine, chemical industry and agriculture and other fields. The special D-lactic acid can be widely used in the fields of medicine, chemical industry and agriculture. Polylactic acid synthesized with D-lactic acid as a monomer has become the most promising green environmental protection material in the 21st century due to its good biodegradability and other excellent use characteristics (such as transparency, thermoplasticity, product safety, etc.). In addition, herbicides such as "Puma" and "Weiba", which are made of high-purity D-lactic acid, are new types of low-toxicity pesticides, which can effectively kill weeds and insects and are economical and reliabl...

Claims

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Application Information

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IPC IPC(8): C07C59/08C07C51/42
Inventor 方晓江刘经伟马江锋陈韶辉杨爱武李晓强柏基业
Owner SINOPEC YANGZI PETROCHEM
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