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Method for detecting concentration of acrylamide in solution

A technology of acrylamide and concentration, applied in the field of chemical detection, can solve the problems of cumbersome processing, long analysis time, high cost of instruments and medicines, and achieve the effects of simple and convenient operation, improved sensitivity and reduced detection cost

Inactive Publication Date: 2015-10-07
GUANGXI TEACHERS EDUCATION UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, these methods have disadvantages such as cumbersome pretreatment process, long analysis time, high cost of instruments and medicines, etc.

Method used

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  • Method for detecting concentration of acrylamide in solution
  • Method for detecting concentration of acrylamide in solution

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Embodiment 1

[0038] A method for detecting the concentration of acrylamide in a solution, comprising:

[0039] Use the three-electrode system to detect acrylamide in the liquid to be detected by square wave voltammetry, and obtain the concentration of acrylamide in the liquid to be detected according to the linear equation of acrylamide, wherein the working electrode in the three-electrode system is characterized by single-stranded DNA A modified gold electrode, the sequence of the characteristic single-stranded DNA is 5'-AAA AAA AAA GGA AAA AAA AA-(CH 2 )6 -SH-3'. The three-electrode system consists of a working electrode, a reference electrode and an auxiliary electrode.

[0040] The present invention uses square wave voltammetry to detect acrylamide in the liquid to be detected. In square wave voltammetry, the baseline potential is gradually swept from the initial potential to the termination potential in a stepwise manner, and a square wave voltage is superimposed. The basic voltage i...

Embodiment 2

[0060] Electrochemical workstation: CHI760E;

[0061] Use the three-electrode system to detect acrylamide in the liquid to be detected by square wave voltammetry, and obtain the concentration of acrylamide in the liquid to be detected according to the linear equation of acrylamide, wherein the working electrode in the three-electrode system is characterized by single-stranded DNA Modified gold electrode, the sequence of characteristic single-stranded DNA is 5'-AAA AAA AAA GGA AAA AAA AA-(CH 2 ) 6 -SH-3'.

[0062] Buffer solution: pH value is 7.0, concentration is 0.2mol / L phosphate buffer solution.

[0063] Standard stock solution: Acrylamide standard solution with a concentration of 2 μmol / L; Dilute the standard stock solution with buffer solution to obtain the required concentration of acrylamide standard solution.

[0064] Treatment of gold electrodes before modification: place the gold electrodes in turn on polishing cloths containing polishing powders with particle siz...

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Abstract

The invention provides a method for detecting the concentration of acrylamide in a solution, the method comprises the steps of using a three-electrode system to detect the acrylamide in the solution to be detected through a square wave voltammetry, obtaining the concentration of the acrylamide in the solution to be detected according to the linear equation of acrylamide, wherein the working electrode in the three-electrode system is a gold electrode which is modified by characteristic single-stranded DNA, and the DNA sequence is 5minute-AAAAAAAAA GGAAAAAAAAA-(CH2) 6-SH-3minute. According to the method provided by the invention, the square wave voltammetry is utilized for high sensitivity detection of the acrylamide, the detection limit of acrylamide concentration can reach 1mo1 / L, the method is simple in operation, quick in detection, high in sensitivity and good in selectivity, and has a broad application prospect.

Description

technical field [0001] The invention belongs to the technical field of chemical detection, and in particular relates to a method for detecting the concentration of acrylamide in a solution. Background technique [0002] Acrylamide has mutagenic effects in both in vivo and in vitro tests, and can cause gene mutations and chromosomal abnormalities in mammalian somatic and germ cells, such as micronucleus formation, sister chromatid exchange, polyploidy, aneuploidy, and others Abnormal mitosis, etc., dominant lethal test was positive. It was also proved that the metabolite glycidamide of acrylamide is its main mutagenic active substance. Animal experiments have found that acrylamide can cause tumors in various organs of rats, including mammary gland, thyroid gland, testis, adrenal gland, central nervous system, oral cavity, uterus, and pituitary gland. The International Agency for Research on Cancer (IARC) evaluated its carcinogenicity in 1994, and listed acrylamide as a type...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/48
Inventor 肖琦黄珊卢双燕盛家荣黄初升
Owner GUANGXI TEACHERS EDUCATION UNIV
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