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Micron-sized biological material paraffin sectioning method

A biological material and paraffin sectioning technology, applied in the biological field, can solve the problems of inability to slice, easily deform the material, and discontinue the slice, and achieve the effect of overcoming the easy deformation of the material, facilitating popularization and application, and simple and effective fixation.

Active Publication Date: 2015-09-23
CHONGQING MEDICAL UNIVERSITY
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  • Claims
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Problems solved by technology

However, in biological research, slicing usually encounters micron-sized biological materials that cannot be seen by the naked eye, such as cell clusters, animal embryos before implantation, etc. The material is easily deformed, the slices are discontinuous, or there are cavities, resulting in inability to slice or incomplete slices. At present, there has been no ideal paraffin section method for micron-scale biological materials. Researchers can only use electron microscopes that require high costs and equipment. Observation methods that limit the study of micron-scale biomaterials

Method used

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  • Micron-sized biological material paraffin sectioning method
  • Micron-sized biological material paraffin sectioning method
  • Micron-sized biological material paraffin sectioning method

Examples

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Embodiment 1

[0037] Embodiment 1 Paraffin section of mouse preimplantation embryo

[0038]Embryos with a diameter of 30-50um that have not been removed from the oviduct or uterine cavity of the mouse were washed with 1×PBS to remove tissue fragments, red blood cells and other impurities as much as possible. 2-cell and 4-cell stage mouse embryos before burying image 3 shown.

[0039] Paraffin sections were then prepared as follows:

[0040] (1) Take two slides and add two drops of fetal bovine serum and one drop of Bouin's fixative respectively, use a microrotary pipette connected to a glass capillary with a diameter of 100-150um to suck up the tissue to be sliced, and transfer one drop of fetal bovine serum into it After gently blowing and stirring, suck it out, transfer it to the second drop of serum, suspend the tissue to be sliced ​​in fetal bovine serum, and obtain a mixture of mouse embryos and serum, which is convenient for subsequent operations;

[0041] (2) The microrotary pipe...

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Abstract

The invention belongs to the field of biotechnology and particularly relates to a micron-sized biological material paraffin sectioning method. The method comprises the following steps of fixing a micron-sized biological material, i.e. suspending the micron-sized biological material in serum to obtain a mixture of the micron-sized biological material and serum; slowly filling the mixture of the micron-sized biological material and serum into Bouin fixing liquid to obtain a precipitate wrapping the micron-sized biological material; dehydrating the obtained precipitate to obtain a pre-processed material; performing conventional paraffin embedding and sectioning on the obtained pre-processed material. According to the micron-sized biological material paraffin sectioning method provided by the invention, the micron-sized biological material can be simply and effectively fixed, the defects that deformation is easily caused, sectioning is not continuous, or existence of cavities causes incomplete sectioning and the phenomenon that sectioning cannot be performed when the micron-sized biological material is sectioned are overcome, the sectioning thickness can be up to 5 microns. The method is simple in operation, special equipment is not required, a reagent is easy to obtain, and the method is convenient to popularize and use.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a method for paraffin sectioning micron-scale biological materials. Background technique [0002] Paraffin section is the most widely used method in conventional histological preparation technology, which has the characteristics of simple operation and low price. It is the main method for observing and judging the morphological changes of cell tissues, and it has also been widely used in the research of many other disciplines. [0003] Living cells or tissues are mostly colorless and transparent, and there is a lack of contrast between various tissues and various structures in the cells, and it is difficult to distinguish them clearly under a general light microscope; tissues will die soon after leaving the body and produce tissue corruption , Lose the original normal structure. Therefore, the tissue should be fixed, paraffin-embedded, sectioned and stained to avoid cell...

Claims

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Application Information

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IPC IPC(8): G01N1/36G01N1/28
Inventor 谭冬梅谭毅张倩杨根岭赖国旗潘永全
Owner CHONGQING MEDICAL UNIVERSITY
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