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Semen hoveniae extracts with liver protection function and extraction and separation method and application thereof

A separation method and technology of Fructus Fructus, applied in the direction of organic active ingredients, medical preparations containing active ingredients, plant/algae/fungus/moss components, etc., can solve the problem that the medicinal effect material basis of Fructus Fructus is not clearly proposed. , to achieve the effect of good medicinal prospect, strong pharmacological effect and simple preparation process

Active Publication Date: 2015-09-16
JINAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0012] Up to now, the material basis of the medicinal effect of Hovenia dulcis in treating hepatitis has not been clearly proposed at home and abroad.

Method used

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  • Semen hoveniae extracts with liver protection function and extraction and separation method and application thereof
  • Semen hoveniae extracts with liver protection function and extraction and separation method and application thereof
  • Semen hoveniae extracts with liver protection function and extraction and separation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] Embodiment 1, the preparation of total flavonoid glycosides extract of Hovenia dulcis Fructus

[0041] Take 1Kg of the Hovenia dulcis medicinal material, add 70% ethanol to reflux and extract twice, combine the alcohol extracts, recover under reduced pressure until there is no alcohol smell, centrifuge (2000 rpm, 15 minutes), and apply the supernatant to the processed After being adsorbed by the D101 macroporous adsorption resin column, it was eluted successively with 3 liters of water and 5 liters of 50% ethanol, and the 50% ethanol eluate was collected and evaporated to dryness under reduced pressure to obtain 17 g of Hovenia dulcis fruit extract. After determination, the mass content of total flavone carbon glycosides is 73%.

Embodiment 2

[0042] Embodiment 2, preparation of Hovenia dulcis Fructus total flavonoid glycosides extract

[0043] Hovenia dulcis medicinal material 1Kg, add 80% ethanol to reflux and extract 3 times, combine the ethanol extracts, recover under reduced pressure until there is no alcohol smell, add water to suspend, centrifuge (2000 rpm, 15 minutes), and put the supernatant on the The treated polyamide column was eluted with 3 liters of water and 5 liters of 50% ethanol in sequence, and the 50% ethanol eluate was collected and evaporated to dryness under reduced pressure to obtain 22 g of Hovenia dulcis fruit extract. After determination, the mass content of total flavone carbon glycosides is 56%.

Embodiment 3

[0044] Embodiment 3, the preparation of total flavonoid glycosides extract of Hovenia dulcis Fructus

[0045] Get Hovenia dulcis medicinal material 1Kg, add water and reflux to extract 2 times, centrifuge (2000 rev / min, 15 minutes), supernatant is loaded on the D101 macroporous adsorption resin column after absorbing, successively with 2 liters of water, 2 liters of 20% ethanol, Elute with 5 liters of 50% ethanol, collect the 50% ethanol eluate, evaporate to dryness under reduced pressure, add 10 times the amount of 95% ethanol to the obtained extract, heat and reflux for extraction for 1 hour, and suction filter while it is hot, and vacuum dry the filtrate after recovering ethanol. That is, 21 g of Hovenia dulcis fruit extract was obtained. After determination, the mass content of total flavone carbon glycosides is 58%.

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Abstract

The invention discloses semen hoveniae extracts with a liver protection function and an extraction and separation method and application thereof. The extracts comprise, by mass, 10-99% of total flavone C-glycoside, wherein the main flavone C-glycoside comprises six kinds of flavone C-glycoside including vitexin 2''-O-beta-D-glucopyranoside, spinosin, poloretin-3', 5'-di-C-beta-D-glucopyranoside, 6'' '-acetyl spinosin, 6'' '-p- hydroxyl-cinnamoyl-spinosin and 6'' '- sinapoyl-spinosin. The total flavone C-glycoside is extracted from semen hoveniae and used for preventing and treating acute and chronic hepatitis and alcoholic liver disease; compositions at effective parts are accurate; toxicity is small; the pharmacological action is high, and a good medicine prospect is achieved. The extracts and acceptable agents on pharmacy are adopted to be prepared into various dosage forms through a conventional method, like tablets, pills, capsules and granules. The dosage is 200-800 mg / each person / day.

Description

technical field [0001] The invention belongs to the field of natural medicines, and in particular relates to an extract of dulcis dulcis dulcis with hepatoprotective effect and its extraction and separation method and application. Background technique [0002] Liver disease is a common disease that seriously threatens human health. According to the etiology, it can be divided into viral hepatitis, alcoholic hepatitis, non-alcoholic fatty liver, autoimmune hepatitis, drug-toxic hepatitis, genetic metabolic liver disease and other infectious factors. caused by hepatitis. [0003] Alcoholic liver disease (ALD) is a liver disease caused by long-term excessive drinking, which causes fatty degeneration, necrosis and regeneration of liver cells through ethanol and its metabolite acetaldehyde, including alcoholic fatty liver (Alcoholic fatty liver ), alcoholic hepatitis (Alcoholic hepatitis), liver fibrosis (Alcoholic fibrosis), liver cirrhosis (Alcoholic cirrhosis), etc. [0004]...

Claims

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Application Information

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IPC IPC(8): A61K36/72A61K31/7048A61K31/7034A61P1/16
Inventor 张晓琦叶文才高美华李药兰徐方方李华
Owner JINAN UNIVERSITY
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