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Method for extracting platycodin

An extraction method and a technology for platycodon saponins are applied in the field of purifying platycodon saponins and extraction, which can solve the problems of low purity and low extraction rate of platycodon saponins, and achieve the effects of high extraction efficiency, easy industrial production and simple operation.

Inactive Publication Date: 2015-09-16
NANJING ZELANG MEDICAL TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] At present, there are many technical documents and patents related to the extraction of platycodon saponins in our country, which mainly adopt solvent extraction and ultrasonic extraction. The platycodon saponins prepared by these two methods not only have low extraction rate, but also have low purity.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Get fresh platycodon grandiflorum, dry, pulverize, and pass through a 20-mesh sieve. Weigh 50 grams of bellflower powder and put it into an extractor, add 25 milliliters of water to soak for 20 minutes, then add 0.25 grams of pectinase reagent, and enzymolyze it for 2 minutes at a temperature of 30 ° C, collect the raw materials for enzymolysis, and then Add 250 milliliters of 60% ethanol aqueous solution for reflux extraction, extract the filter residue twice, combine the filtrate for 3 times, concentrate under reduced pressure to form an extract, dissolve the gained extract, and pour the solution into the X-5 macroporous adsorption resin column, First wash away the sugar with 2 times column volume of water, then wash away some impurities with 2 times column volume and 10% ethanol, and finally elute with 2 times column volume and 60% ethanol, collect the ethanol eluate, concentrate under reduced pressure, and dry, that is Total saponins were obtained. The content of t...

Embodiment 2

[0025] Get fresh platycodon grandiflorum, dry, pulverize, and pass through a 40-mesh sieve. Weigh 50 grams of bellflower powder and put it into an extractor, add 30 ml of water to soak for 40 minutes, then add 0.5 grams of pectinase, and enzymolyze it for 3 minutes at a temperature of 40°C, collect the raw materials for enzymolysis, and then add 250 milliliters of 60% ethanol aqueous solution was refluxed and extracted, the filter residue was extracted twice, the filtrate was combined for three times, concentrated under reduced pressure and concentrated under reduced pressure to form an extract, the gained extract was dissolved, and the solution was poured into a D3520 macroporous adsorption resin column, First use 3 times the column volume to wash away the sugar, then use 4 times the column volume and 15% ethanol to wash away some impurities, and finally use 4 times the column volume and 65% ethanol to elute, collect the ethanol eluate, concentrate it under reduced pressure, a...

Embodiment 3

[0027] Get fresh platycodon grandiflorum, dry, pulverize, and pass through a 60-mesh sieve. Weigh 50 grams of bellflower powder and put it into the extractor, add 50 ml of water to soak for 60 minutes, then add 1 g of biological enzyme reagent, and enzymolyze it for 4 minutes at a temperature of 45 ° C, collect the raw materials for enzymolysis, and then add 250 milliliters of 60% ethanol aqueous solution was refluxed and extracted, and the filter residue was extracted 3 times, and the filtrate was combined for 4 times, concentrated under reduced pressure to become an extract, and the gained extract was dissolved, and the solution was poured into an AB-8 macroporous adsorption resin column. Wash away the sugar with 4 times column volume of water, then wash away some impurities with 5 times column volume and 20% ethanol, and finally elute with 5 times column volume and 70% ethanol, collect the ethanol eluate, concentrate under reduced pressure, and dry, that is Total saponins w...

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PUM

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Abstract

The invention discloses a method for extracting platycodin. The method comprises the following steps that firstly, the platycodon grandiflorum is dried, crushed and screened; secondly, a certain amount of platycodon grandiflorum powder is weighed and wetted through added water, and bio-enzyme reagent with the weight accounting for 0.5%-2% of the weight of the platycodon grandiflorum powder is added for carrying out enzymolysis processing; thirdly, after enzymolysis is finished, the ethanol water is added for reflux extraction, and the extraction solution is obtained; fourthly, the extraction solution is filtered, filter residues are repeatedly extracted, the extraction solution is combined, and vacuum concentration is carried out so that the extract can be formed; fifthly, the extract is dissolved, the dissolved solution is eluted through a macroporous resin column, the eluant is collected for vacuum concentration and drying, and then the platycodin is obtained. By means of the method for preparing the platycodin, the technology is easy to operate, the production cost is low, and the purity of obtained products is high.

Description

[0001] The invention belongs to the field of biological technology, and relates to a method for extracting and purifying platycodon saponin by combining biological enzymolysis technology and macroporous adsorption resin. Background technique [0002] Platycodon grandiflorus (scientific name: Platycodon grandiflorus, English: Balloon Flower), alias burden flower, bell flower, mitral flower, Campanulaceae Campanulaceae plant, perennial herb, leaves ovate or ovate-lanceolate, flowers dark blue or dark purple White, it can be used as an ornamental flower; Korean people use it as a wild vegetable; its root can be used as medicine, which has the functions of relieving cough and phlegm, clearing the lungs, and draining pus. It is commonly used in traditional Chinese medicine. [0003] The chemical composition contained in platycodon is as follows: the root contains a variety of saponins, and 18 kinds of triterpene saponins have been separated so far, they are; platycodin A, C, D, D2,...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/34
Inventor 刘东锋杨成东
Owner NANJING ZELANG MEDICAL TECH
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