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A kind of method that utilizes uplc-q-tof/ms technology to measure oligomeric guluronic acid rapidly

A technology for guluronic acid and guluronic acid monosaccharide, which is applied in the detection field and can solve the problems of complex steps, long time consumption, and inability to meet the requirements of rapid analysis and detection.

Inactive Publication Date: 2017-07-07
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This method takes a long time and has complicated steps, which cannot meet the requirements of rapid analysis and detection

Method used

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  • A kind of method that utilizes uplc-q-tof/ms technology to measure oligomeric guluronic acid rapidly
  • A kind of method that utilizes uplc-q-tof/ms technology to measure oligomeric guluronic acid rapidly
  • A kind of method that utilizes uplc-q-tof/ms technology to measure oligomeric guluronic acid rapidly

Examples

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Embodiment 1

[0017] Example 1 Detection of oligomeric guluronic acid in fucoidan hydrolyzate

[0018] 1. Standard sample: guluronic acid monosaccharide (G1), guluronic acid disaccharide (G2), guluronic acid triose (G3), guluronic acid tetraose (G4).

[0019] 2. Sample pretreatment: Take 0.1g of fucoidan hydrolyzate and add 1mL of ultrapure water, vortex to mix and dissolve, pass through a 0.22 μm filter membrane at room temperature, and wait for the test.

[0020] 3. Chromatographic conditions: chromatographic column ACQUITY UPLC CSH C18 Column, 2.1 mm×100 mm, 1.7 μm, mobile phase is acetonitrile phase A and 0.1 % formic acid phase B, flow gradient elution program: 0-1 min, 2 % A+ 98 %B; 1-15 min, 2%A+98%B - 60%A+40%B; 15-17 min, 60%A+40%B - 100%A, flow rate 0.3 mL / min, column temperature : 45°C, injection volume 1 μL.

[0021] 4. Mass spectrometry conditions: mass spectrometer WATERS MALDI SYNAPT-Q TOF / MS, electrospray ion source, negative ion scanning mode. Ion source temperature 100°...

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Abstract

The invention discloses a method for rapidly determining oligomeric guluronic acid by using UPLC-Q-TOF / MS technology, which belongs to the technical field of ultra-high performance liquid chromatography-quadrupole tandem time-of-flight mass spectrometry UPLC-Q-TOF / MS detection. The invention quickly measures the oligoguluronic acid through the steps of preparation of standard products, sample pretreatment, chromatographic conditions, mass spectrometry conditions, establishment of a qualitative and quantitative method for the oligomeric guluronic acid, and the like. The present invention detects oligomeric guluronic acid quickly and accurately, and provides the characteristics of parent ions and fragment ions of oligomeric guluronic acid in mass spectrometry, thereby not only realizing the detection of guluronic acid monolayers in samples Accurate qualitative and quantitative analysis of sugars to tetrasaccharides. At the same time, the unknown oligomeric guluronic acid that may be contained in the sample can be speculated and analyzed based on the mass spectrometry information.

Description

technical field [0001] The present invention relates to a detection method of oligomeric guluronic acid (G1-G4), in particular to a detection method of oligomeric guluronic acid (G1-G4) using ultra-high performance liquid chromatography-quadrupole tandem time-of-flight mass spectrometry (UPLC Q TOF / MS). A rapid qualitative and quantitative method for glucuronic acid belongs to the technical field of detection. Background technique [0002] The detection method of oligomeric guluronic acid involved in the present invention can be used in the quality control of related medicines, health care products and the like. Oligomeric guluronic acid is a hydrolyzed product of alginate. Studies have shown that oligomeric alginate has the effects of lowering blood pressure, lowering blood lipids, anti-oxidation, and anti-inflammation. It has been used in the treatment of cardiovascular diseases. At present, there are few reports on the detection method of oligoguluronic acid. In the cur...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/88G01N30/60G01N30/72G01N30/34
Inventor 张爽陶冠军秦昉陈洁
Owner JIANGNAN UNIV
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