Composite microbial agent for prevention and treatment of tomato root knot nematode disease and application thereof
A compound microbial inoculant, tomato root-knot nematode technology, applied in the directions of microorganisms, biocides, nematicides, etc., can solve the problems of single strain, can not further improve the control efficiency, single biocontrol mechanism, etc., to reduce damage and Suppressing, beneficial to human health, effects that do not require special equipment
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Embodiment 1
[0026] The invention relates to a composite microbial agent, the active ingredients of which are composed of Glomus versiforme (G.v) and Bacillus subtilis (BS).
[0027] A biocontrol bacterial agent for preventing and treating tomato root-knot nematode is prepared by using the composite microbial bacterial agent.
[0028] The method for the prevention and treatment of tomato root-knot nematode by biocontrol agent, at first, in the tomato seedling cultivation stage, inoculate Glomus G.v in the seedling cultivation soil, the inoculation dose is 2000IPU / strain; Again, in the tomato 3-4 leaf transplanting stage , seedling stage and flowering and fruit-setting stage, inoculate described Bacillus subtilis BS in 3 times, and BS inoculation dose is that the concentration of fermented liquid is 10 9 cfu / ml, 200ml / plant. The inoculation method of Bacillus subtilis is to make 4 small holes in the tomato root circle, and use the root irrigation method to inoculate.
Embodiment 2
[0030]The invention relates to a composite microbial agent, the active ingredients of which are composed of Glomus versiforme (G.v) and Bacillus subtilis (BS).
[0031] A biocontrol bacterial agent for preventing and treating tomato root-knot nematode is prepared by using the composite microbial bacterial agent.
[0032] The method for the prevention and treatment of tomato root-knot nematode by biocontrol agent, at first, in the tomato seedling cultivation stage, inoculate Glomus surface G.v in the seedling cultivation soil, the inoculation dosage is 1000IPU / strain; Again, in the tomato 3-4 leaf transplanting stage , seedling stage and flowering and fruit-setting stage, inoculate described Bacillus subtilis BS in 3 times, and BS inoculation dose is that the concentration of fermented liquid is 10 9 cfu / ml, 500ml / plant. The inoculation method of Bacillus subtilis is to make 4 small holes in the tomato root circle, and use the root irrigation method to inoculate.
Embodiment 3
[0034] The invention relates to a composite microbial agent, the active ingredients of which are composed of Glomus versiforme (G.v) and Bacillus subtilis (BS).
[0035] A biocontrol bacterial agent for preventing and treating tomato root-knot nematode is prepared by using the composite microbial bacterial agent.
[0036] The method for the prevention and treatment of tomato root-knot nematode by biocontrol agent, at first, in the tomato seedling cultivation stage, inoculate Glomus surface G.v in the seedling cultivation soil, the inoculation dose is 5000IPU / strain; , seedling stage and flowering and fruit-setting stage, inoculate described Bacillus subtilis BS in 3 times, BS inoculation dosage is fermented liquid 10 9 cfu / ml, 300ml / plant. The inoculation method of Bacillus subtilis is to make 4 small holes in the tomato root circle, and use the root irrigation method to inoculate.
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