Method using living cell sensing instrument for on-line monitoring of culture solution conductivity for production

A technology of cell culture and culture fluid, applied in the field of microbial culture

Inactive Publication Date: 2015-08-26
上海国佳生化工程技术研究中心有限公司
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Problems solved by technology

The existing bio-fermentation method is limited to the laboratory, and the production of paclitaxel can only be increased by enzymes

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  • Method using living cell sensing instrument for on-line monitoring of culture solution conductivity for production

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Embodiment Construction

[0024] The specific implementation of the method for on-line monitoring of the conductivity of the culture medium for production according to the present invention using a live cell sensor will be described in detail below with reference to the accompanying drawings.

[0025] See attached figure 1 , The online detection of the conductivity of the culture medium through the living cell electrode, and the control of the production of paclitaxel by the conductivity, including the following steps:

[0026] (1) Inject a certain amount of culture medium into the mixing tank to maintain the temperature and stirring rate of the mixing tank;

[0027] (2) Pour the cell culture evenly into the stirring tank, and the amount of the cell culture is less than the amount of the medium;

[0028] (3) Collect the conductivity of the culture solution through the living cell electrode of the living cell sensor, record it as the initial conductivity, and determine a reference conductivity range based on the...

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Abstract

The present invention belongs to the microbial cultivation technical field, and discloses a method using a living cell sensing instrument for on-line monitoring of culture solution conductivity for production, a certain amount of a culture medium is injected into a stirring tank, a cell culture is evenly poured into the stirring tank, the amount of the cell culture is less than the amount of the culture medium; the culture solution conductivity is collected by a living cell electrode of the living cell sensing instrument, and recorded as initial conductivity, according to the initial conductivity, a benchmark conductivity range is determined; the culture solution real-time conductivity can be collected in real time; cells are cultured, and Ag < +> ions are added into the culture solution; MeJA solution is added into the culture solution; a cyclodextrin solution is added into the culture solution; the amount of residual sugar in the culture solution is controlled; and a finished product is produced. Through the on-line monitoring of living cell conductivity, the rate for producing taxol from taxus chinensis cells can reach the highest value.

Description

Technical field [0001] The invention belongs to the technical field of microbial culture, in particular to a production method for on-line monitoring of the conductivity of the culture solution by using a live cell sensor. Background technique [0002] Paclitaxel is a complex secondary metabolite in Taxus plants, and it is also the only drug that can promote the polymerization of microtubules and stabilize the polymerized microtubules. [0003] People's methods of obtaining paclitaxel mainly include natural extraction and biological fermentation. Natural extraction methods have low yield, high work intensity and serious pollution. The key of the biological fermentation method is the control of the growth rate of the yew cells. The existing biological fermentation methods are limited to laboratories, and can only increase the production of paclitaxel through enzymes. Summary of the invention [0004] In order to solve the above-mentioned technical problems, the present invention pr...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P17/02C12Q1/02
Inventor 庄英萍张渭张建文林雨萌秦天王泽建郭美锦储炬张嗣良
Owner 上海国佳生化工程技术研究中心有限公司
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