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Application of Pedunsaponin A and Pedunsaponin C in preparation of molluscacide

A technology of kudzu saponin and molluscicide, which is applied in the field of kudzu saponin for the preparation of molluscicide, which can solve the problems of no report on the molluscicide activity of kudzu saponin compound, no report on the molluscicide mechanism of kudzu, and achieve low toxicity , Environmentally friendly, obvious poisonous effect

Active Publication Date: 2015-08-26
四川明峰洋生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the prior art, there are only sporadic reports on the use of kudzu for molluscicide, but there is no report on the molluscicide mechanism, molluscicide components and molluscicide specific experimental data of kudzu, and there is no further information about these two specific species. Report on molluscicidal activity of picradzurin compound

Method used

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  • Application of Pedunsaponin A and Pedunsaponin C in preparation of molluscacide
  • Application of Pedunsaponin A and Pedunsaponin C in preparation of molluscacide
  • Application of Pedunsaponin A and Pedunsaponin C in preparation of molluscacide

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] The molluscicidal activity of Pedunsaponin A (i.e. the above-mentioned formula (I) compound) and Pedunsaponin C (i.e. the above-mentioned formula II compound) of the present invention

[0023] 1. Test target

[0024] Apple snail (Pomacea canaliculata): The shell diameter of apple snail is about 15±2mm, which is artificially bred by the pollution-free pesticide research laboratory of Sichuan Agricultural University.

[0025] Oncomelania hupensis Gredler: 7-8 twisted snails were selected and obtained by artificial breeding in the pollution-free pesticide research laboratory of Sichuan Agricultural University.

[0026] 2. Test drug

[0027] Pedunsaponin A and Pedunsaponin C: separated and purified from the crude extract of Pueraria kuerae by medium-pressure C18 column chromatography. Provided by the Pollution-free Pesticide Research Laboratory of Sichuan Agricultural University.

[0028] 50% Niclosamide WP, commercially available.

[0029] Bitter kudzu root water extra...

Embodiment 2 0

[0039] Embodiment two 10% Pedunsaponin A (being above-mentioned formula (I) compound) suspending agent and 10% Pedunsaponin C (being above-mentioned formula II compound) suspending agent field killing apple snail test

[0040] 1. Drugs to be tested

[0041] 10% Pedunsaponin A suspension concentrate and 10% Pedunsaponin C suspension concentrate. Prepared and provided by the Pollution-free Pesticide Research Laboratory of Sichuan Agricultural University.

[0042]50% Niclosamide WP. Market purchase.

[0043] 30% water emulsion of bitter kudzu root water extract. Prepared and provided by the Pollution-free Pesticide Research Laboratory of Sichuan Agricultural University.

[0044] The components of the 10% Pedunsaponin A suspending agent are as follows: 10% by weight of Pedunsaponin A, 6% by weight of sodium dodecylbenzenesulfonate, 8% by weight of alkylphenol polyoxyethylene ether, 1% by weight of xanthan gum, white carbon 20% by weight of black and 100% of water.

[0045] T...

Embodiment 3

[0059] Embodiment three: 10% Pedunsaponin A (being above-mentioned formula (I) compound) suspending agent and 10% Pedunsaponin C (being above-mentioned formula II compound) suspending agent field snail killing test

[0060] 1. Drugs to be tested

[0061] 10% Pedunsaponin A (ie the compound of formula (I) above) and 10% Pedunsaponin C (ie the compound of formula II above) suspension. Prepared and provided by the Pollution-free Pesticide Research Laboratory of Sichuan Agricultural University.

[0062] 50% Niclosamide WP. Market purchase.

[0063] 30% water emulsion of bitter kudzu root water extract. Prepared and provided by the Pollution-free Pesticide Research Laboratory of Sichuan Agricultural University.

[0064] The components of the 10% Pedunsaponin A suspending agent are as follows: 10% by weight of Pedunsaponin A, 6% by weight of sodium dodecylbenzenesulfonate, 8% by weight of alkylphenol polyoxyethylene ether, 1% by weight of xanthan gum, white carbon 20% by weight...

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Abstract

The invention relates to an application of Pedunsaponin A and Pedunsaponin C in preparation of molluscacide, in particular to an application of Pedunsaponin A and Pedunsaponin C in killing of Pomacea canaliculata or Oncomelania hupensis Gredler. The compounds are from plants, have low toxicity and are environment-friendly; the compounds have remarkable toxic activity on injurious snails, the killing rate is up to or higher than 90%, and the compounds have huge development potential in agricultural application; meanwhile, the plants as sources of the two compounds have strong vitality, are abundant in source and facilitate large-scale production and application.

Description

technical field [0001] The invention belongs to the field of molluscicides, and in particular relates to the application of picrinicin to prepare molluscicides. technical background [0002] At present, a large number of documents have proved that saponins have obvious poisonous effect on harmful snails such as apple snails and oncomelanias. Plants such as camellia oleifera, yam, pokeweed and oleander contain a large amount of saponins, which have high activity on snails and have great potential for actual development. Among them, the most comprehensive study is the pentacyclic triterpene saponins isolated and identified from Camellia oleifera, the main active ingredient of which is (3β,16α)-28-oxo-D-pyranosyl- (1→3)-o-β-D-(xyl)pyranosyl-(1→4)-o-6-deoxy-α-L-mannopyranosyl-(1→2-β-D -pyranose-17-methylhydroxy-16,21,22-trihydroxyolean-12-ene. The substance is in 2.5mg·L -1 concentration, the 24h mortality rate of oncomelania was as high as 93.33%. -1 Concentration, 24h onco...

Claims

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Application Information

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IPC IPC(8): A01N43/16A01N25/04A01P9/00
Inventor 陈华保张敏杨春平龚国淑
Owner 四川明峰洋生物科技有限公司
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