Tongling peony tissue culture and rapid propagation seedling raising technical method
A technology for rapid seedling breeding and tissue culture, which is applied in the field of biological agriculture to achieve the effect of improving the cycle
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[0012] A technical method for rapid breeding of Fengdan tissue culture seedlings, comprising the steps of:
[0013] (1) Explant selection: Fengdanya;
[0014] (2) Disinfection of explants: Rinse with running water until clear, then peel off the scales, then soak in 70% alcohol for 30 seconds, then sterilize with 0.1% mercuric chloride for 1 minute, and finally use sterile water Rinse three times, each time 1min;
[0015] (3) Cluster bud induction medium: 1 / 2MS+BA1.0mg / L+GA30.5mg / L+NAA0.1mg / L; culture period is 15 days;
[0016] (4) Rooting medium: 1 / 2MS+BA0.5mg / L+NAA0.5mg / L; culture period is ten to fifteen days;
[0017] (5) Seedling hardening and transplanting: properly open the lid of the culture bottle to facilitate the gas exchange inside and outside the culture bottle. The culture medium on the root is then transplanted in a greenhouse, covered with plastic film to keep moisture; before transplanting, the matrix is all processed through 400 times of carbendazim, aft...
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