Rough type Brucella melitensis attenuated strain and vaccine thereof

A kind of technology of Brucella sheep and rough type, applied in the field of rough type Brucella sheep attenuated strain and vaccine field

Active Publication Date: 2015-08-19
CHINA INST OF VETERINARY DRUG CONTROL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But so far, there are no recombinant Brucella attenuated strains and vaccines obtained by genetic modification at home and abroad to be approved and applied.

Method used

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  • Rough type Brucella melitensis attenuated strain and vaccine thereof
  • Rough type Brucella melitensis attenuated strain and vaccine thereof
  • Rough type Brucella melitensis attenuated strain and vaccine thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0065] Pancreatic Soy Broth (TSB, BD Company, USA) or other suitable culture medium is used as the culture medium for the rough Brucella vaccine strain (RA343). Inject 1% to 2% of the amount into the seed liquid of Brucella melitansis RM57 strain, gradually increase the ventilation at 36-37°C, and ferment and cultivate for 36 hours according to the conventional method. During the cultivation process, you can add For 50% glucose solution, the amount added each time is 1% to 2% of the total amount of the medium.

[0066] After the cultivation is completed, try to use pancreatic soybean agar medium for sampling to conduct a pure test according to the method stipulated in the "Chinese Veterinary Pharmacopoeia", and it is pure. At the same time, take samples and count live bacteria with TSA for reference during concentration.

[0067] Add 0.1% to 0.2% of the total amount of sodium carboxymethyl cellulose to the bacteria liquid that has passed the pure inspection to precipitate the...

Embodiment 2

[0072] Product testing

[0073] The relevant inspection methods involved in the present invention are carried out according to the method of "Chinese Veterinary Pharmacopoeia"

[0074] (1) Physical properties The vaccine should be loose and fast, and can dissolve rapidly within 1 minute after adding the diluent;

[0075] (2) The purely tested vaccines are purely free of other bacteria;

[0076] (3) Safety inspection Dilute the vaccine to contain 1×10 per 1ml 9 CFU live bacteria, subcutaneously inoculate 5 Kunming mice of 18-20 g in the groin, each 0.25 ml / only, observe for 6 days, all of them are healthy and alive (results are shown in Table 1);

[0077] Table 1 Safety inspection results of live brucellosis vaccine (RM57 strain)

[0078]

[0079] -: Healthy survival, no abnormality at the injection site; +: Death or morbidity.

[0080] (4) Viable bacteria count Use TSA medium to count the viable bacteria to count the viable bacteria of the vaccine, and the number of via...

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PUM

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Abstract

The invention relates to a rough type Brucella melitensis attenuated strain and a vaccine thereof. The rough type Brucella melitensis attenuated strain RM57 is screened by using an antibiotics and M factor serum combined domesticating and screening technology. The safety of the rough type attenuated strain is remarkably improved, but the favorable immune effect for Brucella melitensis is stilled retained. If the attenuated strain is prepared into a Brucella melitensis vaccine, the situation that a Brucella melitensis vaccine immunized animal and a wild strain infected animal are difficult to distinguish is changed, and the safety of the existing vaccine is effectively improved.

Description

Technical field [0001] The invention relates to an attenuated strain of Brucella melis rough-type and a vaccine thereof, belonging to the field of veterinary biological products. technical background [0002] Brucellosis (brucellosis) is a zoonotic disease characterized by abortion and fever caused by Brucella or Brucella, which seriously threatens the life and health of humans and various animals. . This disease not only has serious harm to the reproduction and production performance of animals, but more importantly, people are often difficult to cure after being infected with Brucella, thus causing serious public health problems. Elimination of brucellosis has therefore been one of the most important goals of public health programs in countries where Brucella is endemic. At present, the main method to eliminate the disease worldwide is the combination of culling and immunization. For China, where the occurrence of brucellosis is relatively severe, due to the high cost of...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A61K39/10A61P31/04C12R1/01
Inventor 丁家波朱良全蒋卉王楠王芳毛开荣宁宜宝李慧姣张广川张阁彭永
Owner CHINA INST OF VETERINARY DRUG CONTROL
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