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Growth promoting rhizobacteria of strain of semen euryales and application thereof as well as microbial inoculum

A technology for promoting the growth of bacteria and Gorgon root, applied in the field of agricultural microorganisms, to achieve the effect of promoting synthesis, excellent strain resources, and increasing germination rate

Active Publication Date: 2015-08-19
RESEARCH INSTITUTE OF TSINGHUA UNIVERSITY IN SHENZHEN +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the particularity of the water body environment, there are great differences between aquatic vegetables and dryland vegetables in the selection of bacterial strains and the use of bacterial agents. At present, there is no specific growth-promoting bacterial agent for aquatic plants such as Gorgon.

Method used

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  • Growth promoting rhizobacteria of strain of semen euryales and application thereof as well as microbial inoculum

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Example 1: Screening and identification of growth-promoting bacteria in the rhizosphere of Gorgon fruit

[0032] 1. Screening process

[0033] A total of 236 bacterial isolates were isolated from the rhizosphere soil samples of the "Shuibaxian" planting base in Chenghu Agricultural Park, Suzhou, by gradient dilution method, and were respectively picked into inorganic phosphorus medium, organic phosphorus On the culture medium, potassium solution medium and skimmed milk medium, the culture medium was covered with a layer of water agar to simulate the hypoxic environment in the paddy field as much as possible. After culturing for 5-7 days, select bacterial isolates that can form a transparent circle around the colony, screen out phosphorus bacteria, potassium bacteria and protease-producing bacteria, and keep them for future use after repeated purification. The above strains were further screened by the method of green preservation and radish cotyledon weight gain method...

Embodiment 2

[0053] Embodiment 2: application and preparation of microbial bacterial agent

[0054] Activate the growth-promoting bacterium Q67 in the rhizosphere of Gorgon fruit and inoculate it into the NA medium to prepare the seed solution, inoculate it into the fermentation medium at a ratio of 5%, culture it with shaking for 48 hours, and adjust the bacterial concentration to about 10 9 CFU / mL.

[0055] Liquid microbial agent: take part of the above fermentation broth and dilute it to 5×10 with sterile water 8 CFU / mL, as a liquid microbial inoculum.

[0056] Solid microbial agent: sterilize the carrier (vermiculite: rice husk powder = 4:1) and put it in sterilized plastic bags for later use. Under aseptic conditions, fully mix the above-mentioned part of the fermentation liquid with the carrier , prepared into a solid microbial agent, the number of viable bacteria is about 3.1×10 8 CFU / g.

Embodiment 3

[0057] Embodiment 3: Gorgon seedling stage pot experiment

[0058] (1) Determination of Germination Rate of Gorgon Seeds:

[0059] Add 100 mL of liquid microbial agent to 1 L of deionized water, stir with a magnetic stirrer for about 2 hours, let it stand for 30 minutes, take the supernatant to soak the seeds (20 seeds) for about 1 hour, and set the water soaking control at the same time. Germination of Gorgon seeds was carried out, and each treatment was repeated 3 times. Compared with the control group, the germination rate of Gorgon seeds soaked in liquid microbial inoculum was significantly increased. The germination rate of the seeds soaked in the bacterial agent was about 45% in 7-10 days, and the germination rate of the control was only 30%. It can be seen that the preparation of liquid microbial inoculum by the rhizosphere growth-promoting bacteria Q67 of Gorgon fruit can promote the germination of Gorgon fruit seeds, which further confirms that the rhizosphere growt...

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Abstract

The invention relates to growth promoting rhizobacteria of a strain of semen euryales. The classification and the name of the growth promoting rhizobacteria of the strain of semen euryales are baclicus lincheniformis Q67; the growth promoting rhizobacteria of the strain of semen euryales is preserved in the China Center for Type Culture Collection; the preservation number is CCTCC NO: M2014652. By using the growth promoting rhizobacteria Q67 of the semen euryales, which is provided by the invention, the growth and the development of seedlings of the semen euryales can be obviously promoted, and the content of available nitrogen in a soil environment in which the semen euryales grows is improved. The invention also relates to the application of the growth promoting rhizobacteria of the semen euryales to the preparation of a microbial inoculum, and as results show, the microbial inoculum can obviously improve the germination rate of seeds of the semen euryales.

Description

technical field [0001] The invention relates to the field of agricultural microorganisms, in particular to a rhizosphere growth-promoting bacterium of Gorgon fruit and the application of the bacterium in microbial inoculum. Background technique [0002] Gorgon, also known as: chicken head rice, chicken head lotus, chicken head lotus, thorn lotus root, false lotus root, Hunan root, etc., is a representative aquatic vegetable variety in Suzhou area. It has a long production history and has relatively distinctive characteristics. , the production volume is increasing year by year. Gorgon has high nutritional value and medicinal value, and occupies a relatively high proportion in Suzhou's "water eight immortals" planting area, which is of great economic significance. For a long time, people have used chemical fertilizers and pesticides in the cultivation of Gorgon. Although this can guarantee the yield and quality of Gorgon to a certain extent, it will easily lead to the declin...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/00A01P21/00C12R1/10
CPCA01N63/00C12N1/20C12N1/205C12R2001/10
Inventor 吴秉奇刘淑杰程晓王全龙陈福明芦嵩林张其殿张义刘旭
Owner RESEARCH INSTITUTE OF TSINGHUA UNIVERSITY IN SHENZHEN
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