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Oyster activated peptide-zinc chelate and preparation method and application thereof

A chelate and active peptide technology, applied in the field of oyster active peptide zinc chelate and its preparation, can solve problems such as limited physiological functions, and achieve the effects of improving absorption and utilization, high bioavailability, and low cost

Active Publication Date: 2015-07-29
CHINA NAT RES INST OF FOOD & FERMENTATION IND CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

For example, the patent with the publication number CN 103494214 A discloses a casein phosphopeptide zinc chelate, which is a casein phosphopeptide obtained by enzymatically hydrolyzing casein and inorganic zinc for a chelation reaction to obtain a casein phosphopeptide Zinc chelate, although it can improve the absorption rate of zinc to a certain extent, but the physiological function of casein phosphopeptide as a peptide source is limited

Method used

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  • Oyster activated peptide-zinc chelate and preparation method and application thereof
  • Oyster activated peptide-zinc chelate and preparation method and application thereof
  • Oyster activated peptide-zinc chelate and preparation method and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0039] 1. Preparation and quality inspection of oyster active peptide

[0040] 1.1 Preparation of oyster active peptides

[0041] After crushing 600kg of shelled oysters, add 600kg of water, put them into a colloid mill and grind them into oyster slurry, then add food-grade citric acid to the oyster slurry to adjust the pH value to about 3, stir at 20°C for 1 hour, and centrifuge Remove the supernatant to obtain the oyster slag.

[0042] Mix and stir the above oyster dregs with water at a ratio of 1:1 to water and dregs, adjust the pH to 7-9, heat to 60°C, add 10 U of flavor protease and 10 U of papain per gram of oyster dregs for enzymatic After 6 hours of enzymatic hydrolysis, heat the enzymatic solution to 100°C for about 15 minutes to inactivate the enzyme.

[0043] Use a tubular centrifuge at a speed of 14000r / min to centrifuge the enzymolysis solution after deactivating the enzyme, collect the centrifuged supernatant, and perform membrane filtration with an ultrafiltra...

Embodiment 2

[0093] 1. Preparation and quality inspection of oyster active peptide

[0094] After crushing 600kg of shelled oysters, add 1800kg of water and grind them into oyster slurry, then add food-grade citric acid to the oyster slurry to adjust the pH value to about 5, stir at 30°C for 3 hours, and centrifuge to remove the supernatant , to obtain oyster slag.

[0095] Mix and stir the above oyster dregs with water at a ratio of 3:1 to water and dregs, adjust the pH value to 7-9, heat to 45°C, add 50 U of flavor protease and 50 U of papain per gram of oyster dregs for enzymatic After 4 hours of enzymatic hydrolysis, the enzyme was inactivated.

[0096] Centrifuge the enzymatic hydrolysis solution after deactivating the above enzyme at a speed of 14000r / min, collect the centrifuged supernatant, and perform membrane filtration with an ultrafiltration device with a pore size of 0.06μm, under the conditions of pressure 0.3MPa and temperature 55°C. Oyster Active Peptide Permeate.

[009...

Embodiment 3

[0104] 1. Preparation and quality inspection of oyster active peptide

[0105] After crushing 600kg of shelled oysters, add 3000kg of water and grind them into oyster slurry, then add food-grade citric acid to the oyster slurry to adjust the pH value to about 7, stir at 50°C for 5 hours, and centrifuge to remove the supernatant , to obtain oyster slag.

[0106] Mix and stir the above oyster dregs with water at a ratio of 5:1 to water and dregs, adjust the pH value to 7-9, heat to 60°C, add 100 U of flavor protease and 100 U of papain per gram of oyster dregs for enzymatic After 2 hours of enzymatic hydrolysis, the enzyme was inactivated.

[0107] Centrifuge the enzymatic hydrolysis solution after deactivating the above enzyme at a speed of 16000r / min, collect the centrifuged supernatant, and perform membrane filtration with an ultrafiltration device with a pore size of 0.1μm, under the conditions of pressure 0.4MPa and temperature 60°C. Oyster Active Peptide Permeate.

[01...

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Abstract

The invention provides an oyster activated peptide-zinc chelate and a preparation method and application thereof. The preparation method for the oyster activated peptide-zinc chelate comprises the following steps: preparing a peptide solution from oyster activated peptide, and then performing chelating reaction on the peptide solution and a zinc source to prepare chelated reaction liquid; performing sedimentation on the chelated reaction liquid by an organic solvent to prepare the oyster activated peptide-zinc chelate. Particularly, the mass percentage of components with the molecular weight smaller than 1,000 Da in the oyster activated peptide is over 90 percent. The contents of proteins and peptides in the oyster activated peptide are high, and the molecular weight of the peptides is relatively lower; the oyster activated peptide is low in sensitization, low in bitterness score and good in taste; when the oyster activated peptide is chelated with zinc, the chelating rate is high; the prepared oyster activated peptide-zinc chelate is easy to absorb and use; zinc supplementation can be realized, and various physiological efficacies of the oyster activated peptide can be comprehensively enhanced; the oyster activated peptide-zinc chelate has a good application prospect.

Description

technical field [0001] The invention relates to a zinc peptide chelate, in particular to an oyster active peptide zinc chelate and a preparation method and application thereof. Background technique [0002] Zinc is one of the essential trace elements for the human body. It exists in more than 80 metabolic related enzymes, such as DNA polymerase, alkaline phosphatase, superoxide dismutase, etc., and participates in the synthesis of nucleic acids, proteins, fats, sugars and other substances It plays a vital role in important physiological processes such as human growth and development, reproductive inheritance, etc. Due to zinc intake, metabolism, and excretion disorders, when the human body lacks zinc, growth and development retardation, mental retardation, low immunity, and decreased reproductive function will occur, resulting in a series of diseases. The problem of zinc deficiency is common among residents in our country. According to the survey of 19 provinces, the zinc i...

Claims

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Application Information

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IPC IPC(8): A23J1/04A23L1/305
Inventor 蔡木易谷瑞增鲁军刘艳潘兴昌董哲林峰马勇徐亚光马永庆金振涛陈亮陆路刘文颖魏颖张海欣马涛曹珂璐王憬李国明周明
Owner CHINA NAT RES INST OF FOOD & FERMENTATION IND CO LTD
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