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A method for promoting maturation and artificial fertilization of starfish high-quality eggs

A technology of artificial insemination, starfish, applied in the field of marine life

Inactive Publication Date: 2017-03-22
MARINE FISHERIES RES INST OF ZHEJIANG
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Problems solved by technology

There are no reports at home and abroad on the acquisition of high-quality eggs from starfish and the method of artificial insemination

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  • A method for promoting maturation and artificial fertilization of starfish high-quality eggs

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Embodiment Construction

[0024] The technical solutions of the present invention will be further described in detail below in conjunction with the accompanying drawings and specific embodiments.

[0025] The method for promoting maturation and artificial fertilization of starfish high-quality ovum of the present invention comprises the following steps:

[0026] 1. Cultivation of starfish parents

[0027] 20 randomly selected mature starfish parents (the number of female parents and male parents are determined according to the gonads) are placed in a 100L aquarium for breeding. maintain this temperature. During the cultivation period, feed small zooplankton such as Artemia and small copepods, 10-20g each time (0.5-1g per starfish), once every 2-3 days, change the water 100% every 6 days, Parents are bred for more than a week.

[0028] 2. Collection of starfish gonad tissue

[0029] Use a tapered steel needle with a diameter of 3mm to gently open a small hole on one arm of the starfish, and take out...

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Abstract

The invention provides a method for promoting maturation and artificial fertilization of starfish high-quality eggs, comprising: (1) cultivating starfish parents; (2) collecting starfish male and female gonad tissues; Guanine promotes the ripening of starfish eggs; (4) Artificial fertilization of starfish. The invention establishes the method for obtaining high-quality starfish ovum and artificial fertilization for the first time, and screens out the most suitable maturation-promoting agent and maturation-accelerating time for starfish ova through experiments. The invention has the characteristics of convenient operation, high efficiency and practicability, does not harm parent bodies, and the acquisition of ovum is not restricted by seasons; it has important application value and prospect of popularization and application in the aspects of echinoderm reproductive biology and experimental embryology research.

Description

technical field [0001] The invention belongs to the technical field of marine organisms, and in particular relates to a method for promoting maturation and artificial fertilization of high-quality starfish eggs. Background technique [0002] Starfish is a relatively common invertebrate and the representative clock of echinoderms. It has favorable conditions to become a model animal: relatively small individuals, strong vitality, wide adaptability, and easy breeding under laboratory control conditions; the reproductive cycle is relatively It is short and can be controlled under laboratory conditions; it is required to be able to lay eggs in batches or continuously, with a certain amount of eggs laid; there is no hermaphrodite phenomenon under pollution-free natural conditions. As early as 1882, Erie Erich Mechnikov conducted research on starfish larvae, discovered phagocytes for the first time, and won the 1908 Nobel Prize in Physiology or Medicine. Therefore, starfish is a ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01K61/00
CPCY02A40/81
Inventor 徐冬冬张玉荣楼宝丁跃平
Owner MARINE FISHERIES RES INST OF ZHEJIANG
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