Detection method of shellfish paralytic toxin based on image analysis

Abstract

The invention discloses a method for detecting shellfish paralytic toxin based on image analysis. The method first performs sample pretreatment to prepare a sample solution of shellfish paralytic toxin to be tested; then prepares a standard solution and uses a test strip; Through detection image acquisition, image analysis, calculate the pixel value of the G component in the RGB space of the color image of the detection test strip, and then fit the calibration curve of the test strip to detect shellfish paralytic toxin by the least square method; by analyzing the sample to be tested The sub-image G component pixel values ​​of the control line and detection line of the solution on the test strip are brought into the calibration curve, and then the shellfish paralytic toxin concentration of the sample solution to be tested is calculated. The invention realizes the quantitative detection of shellfish paralytic toxin, and has the advantages of simple operation steps, rapid detection, adaptability to on-site detection and the like.

Description

technical field [0001] The invention relates to a technology for detecting shellfish paralytic toxin, in particular to an image analysis-based detection method for shellfish paralytic toxin. Background technique [0002] Shellfish paralyzing toxin is a kind of neurotoxin, which comes from the toxic algae in red tide. When the toxic algae occurs, shellfish ingest a large amount of toxic algae, which will accumulate toxin in the shellfish. People will be poisoned and even die if they eat it by mistake. At present, domestic shellfish paralytic toxin detection methods usually use mouse bioassay and traditional Elisa kit detection method. The mouse bioassay method is cumbersome to operate, the sensitivity is not high, and the large individual differences lead to low reproducibility, making it difficult to estimate the toxicity. The detection instrument used in the traditional Elisa kit detection method is a microplate reader, which is too large to adapt to on-site detection, and...

AI Technical Summary

Problems solved by technology

The mouse bioassay method is cumbersome to operate, the sensitivity is not high, and the individual differences lead to low reproducibility, making it difficult to estimate the toxicity

The detection instrument used in the traditional Elisa kit detection method is a microplate reader, which is too large to adapt to on-site detection, and there are differences in the enzyme-linked reaction with different incubation times. The time-sharing detection method adopted by the microplate reader resulting in differences in test results between wells

Images